Conducting polymer hydrogels with electrically-tuneable mechanical properties as dynamic cell culture substrates

Show simple item record Ting, Matthew S Vella, Joseph Raos, Brad J Narasimhan, Badri Narayanan Svirskis, Darren Travas-Sejdic, Jadranka Malmström, Jenny 2022-01-19T20:23:27Z 2022-01-19T20:23:27Z 2021-11-1
dc.identifier.citation Materials Science and Engineering C 112559 01 Nov 2021
dc.identifier.issn 0928-4931
dc.description.abstract Hydrogels are a popular substrate for cell culture due to their mechanical properties closely resembling natural tissue. Stimuli-responsive hydrogels are a good platform for studying cell response to dynamic stimuli. Poly(N-isopropylacrylamide) (pNIPAM) is a thermo-responsive polymer that undergoes a volume-phase transition when heated to 32 °C. Conducting polymers can be incorporated into hydrogels to introduce electrically responsive properties. The conducting polymer, polypyrrole (PPy), has been widely studied as electrochemical actuators due to its electrochemical stability, fast actuation and high strains. We determine the volume-phase transition temperature of pNIPAM hydrogels with PPy electropolymerised with different salts as a film within the hydrogel network. We also investigate the electro-mechanical properties at the transition temperature (32 °C) and physiological temperature (37 °C). We show statistically significant differences in the Young's modulus of the hybrid hydrogel at elevated temperatures upon electrochemical stimulation, with a 5 kPa difference at the transition temperature. Furthermore, we show a three-fold increase in actuation at transition temperature compared to room temperature and physiological temperature, attributed to the movement of ions in/out of the PPy film that induce the volume-phase transition of the pNIPAM hydrogel. Furthermore, cell adhesion to the hybrid hydrogel was demonstrated with mouse articular chondrocytes.
dc.language en
dc.publisher Elsevier BV
dc.relation.ispartofseries Materials Science and Engineering C
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject 0903 Biomedical Engineering
dc.subject 0912 Materials Engineering
dc.title Conducting polymer hydrogels with electrically-tuneable mechanical properties as dynamic cell culture substrates
dc.type Journal Article
dc.identifier.doi 10.1016/j.msec.2021.112559
pubs.begin-page 112559 2021-12-16T20:07:06Z
dc.rights.holder Copyright: Elsevier en
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Journal Article
pubs.elements-id 875314
dc.identifier.eissn 1873-0191
pubs.number 112559

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