Inhibition of strigolactone receptors by <i>N</i>-phenylanthranilic acid derivatives: Structural and functional insights.

Show simple item record Hamiaux, Cyril Drummond, Revel SM Luo, Zhiwei Lee, Hui Wen Sharma, Prachi Janssen, Bart J Perry, Nigel B Denny, William A Snowden, Kimberley C
dc.coverage.spatial United States 2022-01-25T20:31:45Z 2022-01-25T20:31:45Z 2018-4
dc.identifier.issn 0021-9258
dc.description.abstract The strigolactone (SL) family of plant hormones regulates a broad range of physiological processes affecting plant growth and development and also plays essential roles in controlling interactions with parasitic weeds and symbiotic fungi. Recent progress elucidating details of SL biosynthesis, signaling, and transport offers many opportunities for discovering new plant-growth regulators via chemical interference. Here, using high-throughput screening and downstream biochemical assays, we identified <i>N</i>-phenylanthranilic acid derivatives as potent inhibitors of the SL receptors from petunia (DAD2), rice (OsD14), and <i>Arabidopsis</i> (AtD14). Crystal structures of DAD2 and OsD14 in complex with inhibitors further provided detailed insights into the inhibition mechanism, and <i>in silico</i> modeling of 19 other plant strigolactone receptors suggested that these compounds are active across a large range of plant species. Altogether, these results provide chemical tools for investigating SL signaling and further define a framework for structure-based approaches to design and validate optimized inhibitors of SL receptors for specific plant targets.
dc.format.medium Print-Electronic
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartofseries The Journal of biological chemistry
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Arabidopsis
dc.subject Petunia
dc.subject Receptors, Cell Surface
dc.subject Arabidopsis Proteins
dc.subject Structure-Activity Relationship
dc.subject Models, Molecular
dc.subject Computer Simulation
dc.subject ortho-Aminobenzoates
dc.subject Oryza
dc.subject chemical biology
dc.subject crystal structure
dc.subject hormone receptor
dc.subject hydrolase
dc.subject inhibition mechanism
dc.subject plant hormone
dc.subject strigolactone
dc.subject strigolactone receptor inhibitor
dc.subject Arabidopsis
dc.subject Arabidopsis Proteins
dc.subject Computer Simulation
dc.subject Models, Molecular
dc.subject Oryza
dc.subject Petunia
dc.subject Receptors, Cell Surface
dc.subject Structure-Activity Relationship
dc.subject ortho-Aminobenzoates
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Biochemistry & Molecular Biology
dc.subject F-BOX PROTEIN
dc.subject GERMINATION
dc.subject ARABIDOPSIS
dc.subject KARRIKIN
dc.subject DWARF14
dc.subject GROWTH
dc.subject 0607 Plant Biology
dc.subject 0601 Biochemistry and Cell Biology
dc.subject Biomedical
dc.subject Basic Science
dc.subject 03 Chemical Sciences
dc.subject 06 Biological Sciences
dc.subject 11 Medical and Health Sciences
dc.title Inhibition of strigolactone receptors by <i>N</i>-phenylanthranilic acid derivatives: Structural and functional insights.
dc.type Journal Article
dc.identifier.doi 10.1074/jbc.ra117.001154
pubs.issue 17
pubs.begin-page 6530
pubs.volume 293 2021-12-15T01:35:51Z
dc.rights.holder Copyright: The author en
pubs.end-page 6543
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't
pubs.subtype research-article
pubs.subtype Journal Article
pubs.elements-id 732173
dc.identifier.eissn 1083-351X
dc.identifier.pii S0021-9258(20)39750-7

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