dc.contributor.author |
Bansal, Amita |
|
dc.contributor.author |
Alsweiler, Jane M |
|
dc.contributor.author |
Oliver, Mark H |
|
dc.contributor.author |
Jaquiery, Anne |
|
dc.contributor.author |
Phua, Hui Hui |
|
dc.contributor.author |
Dragunow, Mike |
|
dc.contributor.author |
de Matteo, Rob |
|
dc.contributor.author |
Harding, Jane E |
|
dc.contributor.author |
Bloomfield, Frank H |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2022-01-25T21:14:35Z |
|
dc.date.available |
2022-01-25T21:14:35Z |
|
dc.date.issued |
2021-2 |
|
dc.identifier.issn |
2040-1744 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/58058 |
|
dc.description.abstract |
Antenatal exogenous glucocorticoids (ANG) are standard management for women at risk of preterm birth but are reputed to impair glucose tolerance in preterm offspring. We compared lambs born preterm (137 days gestation) following labour induced with exogenous glucocorticoids (G-Prem, glucocorticoid-induced preterm group), or with a progesterone synthesis inhibitor (NG-Prem, non-glucocorticoid-induced preterm group), with term-born lambs (Term; 149 days). We assessed glucose tolerance, insulin secretion and sensitivity at 4 and 10 months n = 11-14/group) and pancreatic and hepatic gene and protein expression at 4 weeks post-term (4 weeks; n = 6/group) and 12 months (12 months; n = 12-13/group). NG-Prem had higher plasma glucose concentrations than G-Prem, but not Term, at 4 months (Mean[SEM] mM: NG-Prem = 4.1[0.1]; G-Prem = 3.4[0.1]; Term = 3.7[0.1]; p = 0.003) and 10 months (NG-Prem = 3.9[0.1]; G-Prem = 3.5[0.1]; Term = 3.7[0.1]; p = 0.01). Insulin sensitivity decreased from 4 to 10 months, in NG-Prem but not in Term (Mean[SEM] µmol·ml-1·kg-1·min-1·ng-1, 4 vs. 10 months: NG-Prem = 18.7[2.5] vs. 9.5[1.5], p < 0.01; Term: 12.1[2.8] vs. 10.4[1.5], p = 0.44). At 12 months, β-cell mass in NG-Prem was reduced by 30% vs. G-Prem (p < 0.01) and 75% vs. Term (p < 0.01) and was accompanied by an increased β-cell apoptosis: proliferation ratio at 12 months. At 12 months, pancreatic glucokinase, igf2 and insulin mRNA levels were reduced 21%-71% in NG-Prem vs. G-Prem and 42%-80% vs. Term. Hepatic glut2 mRNA levels in NG-Prem were 250% of those in G-Prem and Term. Thus, induction of preterm birth without exogenous glucocorticoids more adversely affected pancreas and liver than induction with exogenous glucocorticoids. These findings do not support that ANG lead to long-term adverse metabolic effects, but support an effect of preterm birth itself. |
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dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Cambridge University Press (CUP) |
|
dc.relation.ispartofseries |
Journal of developmental origins of health and disease |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
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dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
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dc.subject |
Animals |
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dc.subject |
Sheep |
|
dc.subject |
Humans |
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dc.subject |
Premature Birth |
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dc.subject |
Insulin Resistance |
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dc.subject |
Disease Models, Animal |
|
dc.subject |
Insulin |
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dc.subject |
Blood Glucose |
|
dc.subject |
Glucocorticoids |
|
dc.subject |
Glucose Tolerance Test |
|
dc.subject |
Labor, Induced |
|
dc.subject |
Apoptosis |
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dc.subject |
Cell Proliferation |
|
dc.subject |
Pregnancy |
|
dc.subject |
Female |
|
dc.subject |
Insulin-Secreting Cells |
|
dc.subject |
Preterm birth |
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dc.subject |
insulin secretion |
|
dc.subject |
insulin sensitivity |
|
dc.subject |
steroids |
|
dc.subject |
β-cells |
|
dc.subject |
Animals |
|
dc.subject |
Apoptosis |
|
dc.subject |
Blood Glucose |
|
dc.subject |
Cell Proliferation |
|
dc.subject |
Disease Models, Animal |
|
dc.subject |
Female |
|
dc.subject |
Glucocorticoids |
|
dc.subject |
Glucose Tolerance Test |
|
dc.subject |
Humans |
|
dc.subject |
Insulin |
|
dc.subject |
Insulin Resistance |
|
dc.subject |
Insulin-Secreting Cells |
|
dc.subject |
Labor, Induced |
|
dc.subject |
Pregnancy |
|
dc.subject |
Premature Birth |
|
dc.subject |
Sheep |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Public, Environmental & Occupational Health |
|
dc.subject |
Preterm birth |
|
dc.subject |
steroids |
|
dc.subject |
insulin secretion |
|
dc.subject |
insulin sensitivity |
|
dc.subject |
beta-cells |
|
dc.subject |
GROWTH-FACTOR-I |
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dc.subject |
SHEEP |
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dc.subject |
INHIBITION |
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dc.subject |
EXPOSURE |
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dc.subject |
UNDERNUTRITION |
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dc.subject |
RESTRICTION |
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dc.subject |
PARTURITION |
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dc.subject |
EXPRESSION |
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dc.subject |
INCREASES |
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dc.subject |
SECRETION |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
Effects of preterm birth induced with or without exogenous glucocorticoids on the ovine glucose-insulin axis. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1017/s2040174419000916 |
|
pubs.issue |
1 |
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pubs.begin-page |
58 |
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pubs.volume |
12 |
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dc.date.updated |
2021-12-14T22:54:28Z |
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dc.rights.holder |
Copyright: The author |
en |
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/31937391 |
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pubs.end-page |
70 |
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pubs.publication-status |
Published |
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dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
793332 |
|
dc.identifier.eissn |
2040-1752 |
|
dc.identifier.pii |
S2040174419000916 |
|
pubs.online-publication-date |
2020-1-15 |
|