First transcriptome of the copepod Gladioferens pectinatus subjected to chronic contaminant exposures.

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dc.contributor.author Barrick, Andrew
dc.contributor.author Laroche, Olivier
dc.contributor.author Boundy, Michael
dc.contributor.author Pearman, John K
dc.contributor.author Wiles, Tanja
dc.contributor.author Butler, Juliette
dc.contributor.author Pochon, Xavier
dc.contributor.author Smith, Kirsty F
dc.contributor.author Tremblay, Louis A
dc.coverage.spatial Netherlands
dc.date.accessioned 2022-02-03T20:30:21Z
dc.date.available 2022-02-03T20:30:21Z
dc.date.issued 2021-12-24
dc.identifier.citation Aquatic toxicology 243:106069 Feb 2022
dc.identifier.issn 0166-445X
dc.identifier.uri https://hdl.handle.net/2292/58117
dc.description.abstract Contaminants are often at low concentrations in ecosystems and their effects on exposed organisms can occur over long periods of time and across multiple generations. Alterations to subcellular mechanistic pathways in response to exposure to contaminants can provide insights into mechanisms of toxicity that methods measuring higher levels of biological may miss. Analysis of the whole transcriptome can identify novel mechanisms of action leading to impacts in exposed biota. The aim of this study was to characterise how exposures to copper, benzophenone and diclofenac across multiple generations altered molecular expression pathways in the marine copepod Gladioferens pectinatus. Results of the study demonstrated differential gene expression was observed in cultures exposure to diclofenac (569), copper (449) and benzophenone (59). Pathways linked to stress, growth, cellular and metabolic processes were altered by exposure to all three contaminants with genes associated with oxidative stress and xenobiotic regulation also impacted. Protein kinase functioning, cytochrome P450, transcription, skeletal muscle contraction/relaxation, mitochondrial phosphate translocator, protein synthesis and mitochondrial methylation were all differentially expressed with all three chemicals. The results of the study also suggested that using dimethyl sulfoxide as a dispersant influenced the transcriptome and future research may want to investigate it's use in molecular studies. Data generated in this study provides a first look at transcriptomic response of G. pectinatus exposed to contaminants across multiple generations, future research is needed to validate the identified biomarkers and link these results to apical responses such as population growth to demonstrate the predictive capacity of molecular tools.
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartofseries Aquat Toxicol
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject Benzophenone
dc.subject Copper
dc.subject Diclofenac
dc.subject Ecotoxicology
dc.subject Multigeneration
dc.subject Transcriptome
dc.subject 03 Chemical Sciences
dc.subject 05 Environmental Sciences
dc.subject 06 Biological Sciences
dc.title First transcriptome of the copepod Gladioferens pectinatus subjected to chronic contaminant exposures.
dc.type Journal Article
dc.identifier.doi 10.1016/j.aquatox.2021.106069
pubs.begin-page 106069
pubs.volume 243
dc.date.updated 2022-01-13T18:46:08Z
dc.rights.holder Copyright: 2021 Elsevier B.V. en
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/34968986
pubs.publication-status Published online
dc.rights.accessrights http://purl.org/eprint/accessRights/RetrictedAccess en
pubs.subtype Journal Article
pubs.elements-id 879505
dc.identifier.eissn 1879-1514
dc.identifier.pii S0166-445X(21)00329-5
pubs.number 106069


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