dc.contributor.author |
Harfoot, Rhodri |
|
dc.contributor.author |
Yung, Deborah BY |
|
dc.contributor.author |
Anderson, William A |
|
dc.contributor.author |
Wild, Cervantée EK |
|
dc.contributor.author |
Coetzee, Nicolene |
|
dc.contributor.author |
Hernández, Leonor C |
|
dc.contributor.author |
Lawley, Blair |
|
dc.contributor.author |
Pletzer, Daniel |
|
dc.contributor.author |
Derraik, José GB |
|
dc.contributor.author |
Anderson, Yvonne C |
|
dc.contributor.author |
Quiñones-Mateu, Miguel E |
|
dc.date.accessioned |
2022-02-17T21:57:03Z |
|
dc.date.available |
2022-02-17T21:57:03Z |
|
dc.identifier.citation |
Pathogens 11(1) 10 Jan 2022 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/58257 |
|
dc.description.abstract |
<jats:p>The arrival of SARS-CoV-2 to Aotearoa/New Zealand in February 2020 triggered a massive response at multiple levels. Procurement and sustainability of medical supplies to hospitals and clinics during the then upcoming COVID-19 pandemic was one of the top priorities. Continuing access to new personal protective equipment (PPE) was not guaranteed; thus, disinfecting and reusing PPE was considered as a potential alternative. Here, we describe part of a local program intended to test and implement a system to disinfect PPE for potential reuse in New Zealand. We used filtering facepiece respirator (FFR) coupons inoculated with SARS-CoV-2 or clinically relevant multidrug-resistant pathogens (Acinetobacter baumannii Ab5075, methicillin-resistant Staphylococcus aureus USA300 LAC and cystic-fibrosis isolate Pseudomonas aeruginosa LESB58), to evaluate the potential use of ultraviolet-C germicidal irradiation (UV-C) or dry heat treatment to disinfect PPE. An applied UV-C dose of 1000 mJ/cm2 was sufficient to completely inactivate high doses of SARS-CoV-2; however, irregularities in the FFR coupons hindered the efficacy of UV-C to fully inactivate the virus, even at higher UV-C doses (2000 mJ/cm2). Conversely, incubating contaminated FFR coupons at 65 °C for 30 min or 70 °C for 15 min, was sufficient to block SARS-CoV-2 replication, even in the presence of mucin or a soil load (mimicking salivary or respiratory secretions, respectively). Dry heat (90 min at 75 °C to 80 °C) effectively killed 106 planktonic bacteria; however, even extending the incubation time up to two hours at 80 °C did not completely kill bacteria when grown in colony biofilms. Importantly, we also showed that FFR material can harbor replication-competent SARS-CoV-2 for up to 35 days at room temperature in the presence of a soil load. We are currently using these findings to optimize and establish a robust process for decontaminating, reusing, and reducing wastage of PPE in New Zealand.</jats:p> |
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dc.language |
en |
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dc.publisher |
MDPI AG |
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dc.relation.ispartofseries |
Pathogens |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
1107 Immunology |
|
dc.subject |
1108 Medical Microbiology |
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dc.title |
Ultraviolet-C Irradiation, Heat, and Storage as Potential Methods of Inactivating SARS-CoV-2 and Bacterial Pathogens on Filtering Facepiece Respirators |
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dc.type |
Journal Article |
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dc.identifier.doi |
10.3390/pathogens11010083 |
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pubs.issue |
1 |
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pubs.begin-page |
83 |
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pubs.volume |
11 |
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dc.date.updated |
2022-01-11T10:01:30Z |
|
dc.rights.holder |
Copyright: The author |
en |
pubs.end-page |
83 |
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pubs.publication-status |
Published online |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.elements-id |
879425 |
|
dc.identifier.eissn |
2076-0817 |
|
pubs.online-publication-date |
2022-1-10 |
|