Magnetic Resonance Imaging Correlates of White Matter Gliosis and Injury in Preterm Fetal Sheep Exposed to Progressive Systemic Inflammation.

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dc.contributor.author Galinsky, Robert
dc.contributor.author van de Looij, Yohan
dc.contributor.author Mitchell, Natasha
dc.contributor.author Dean, Justin M
dc.contributor.author Dhillon, Simerdeep K
dc.contributor.author Yamaguchi, Kyohei
dc.contributor.author Lear, Christopher A
dc.contributor.author Wassink, Guido
dc.contributor.author Davidson, Joanne O
dc.contributor.author Nott, Fraser
dc.contributor.author Zahra, Valerie A
dc.contributor.author Kelly, Sharmony B
dc.contributor.author King, Victoria J
dc.contributor.author Sizonenko, Stéphane V
dc.contributor.author Bennet, Laura
dc.contributor.author Gunn, Alistair J
dc.coverage.spatial Switzerland
dc.date.accessioned 2022-03-06T22:04:14Z
dc.date.available 2022-03-06T22:04:14Z
dc.date.issued 2020-11-24
dc.identifier.citation International journal of molecular sciences 21(23) 24 Nov 2020
dc.identifier.issn 1422-0067
dc.identifier.uri https://hdl.handle.net/2292/58457
dc.description.abstract Progressive fetal infection/inflammation is strongly associated with neural injury after preterm birth. We aimed to test the hypotheses that progressively developing fetal inflammation leads to neuroinflammation and impaired white matter development and that the histopathological changes can be detected using high-field diffusion tensor magnetic resonance imaging (MRI). Chronically instrumented preterm fetal sheep at 0.7 of gestation were randomly assigned to receive intravenous saline (control; <i>n</i> = 6) or a progressive infusion of lipopolysaccharide (LPS, 200 ng intravenous over 24 h then doubled every 24 h for 5 days to induce fetal inflammation, <i>n</i> = 7). Sheep were killed 10 days after starting the infusions, for histology and high-field diffusion tensor MRI. Progressive LPS infusion was associated with increased circulating interleukin (IL)-6 concentrations and moderate increases in carotid artery perfusion and the frequency of electroencephalogram (EEG) activity (<i>p</i> < 0.05 vs. control). In the periventricular white matter, fractional anisotropy (FA) was increased, and orientation dispersion index (ODI) was reduced (<i>p</i> < 0.05 vs. control for both). Histologically, in the same brain region, LPS infusion increased microglial activation and astrocyte numbers and reduced the total number of oligodendrocytes with no change in myelination or numbers of immature/mature oligodendrocytes. Numbers of astrocytes in the periventricular white matter were correlated with increased FA and reduced ODI signal intensities. Astrocyte coherence was associated with increased FA. Moderate astrogliosis, but not loss of total oligodendrocytes, after progressive fetal inflammation can be detected with high-field diffusion tensor MRI.
dc.format.medium Electronic
dc.language eng
dc.publisher MDPI AG
dc.relation.ispartofseries International journal of molecular sciences
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Animals
dc.subject Sheep
dc.subject Gliosis
dc.subject Inflammation
dc.subject Magnetic Resonance Imaging
dc.subject Leukoencephalopathies
dc.subject White Matter
dc.subject MRI
dc.subject brain
dc.subject infection
dc.subject inflammation
dc.subject preterm infant
dc.subject Animals
dc.subject Gliosis
dc.subject Inflammation
dc.subject Leukoencephalopathies
dc.subject Magnetic Resonance Imaging
dc.subject Sheep
dc.subject White Matter
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Physical Sciences
dc.subject Biochemistry & Molecular Biology
dc.subject Chemistry, Multidisciplinary
dc.subject Chemistry
dc.subject preterm infant
dc.subject inflammation
dc.subject infection
dc.subject brain
dc.subject MRI
dc.subject 0399 Other Chemical Sciences
dc.subject 0604 Genetics
dc.subject 0699 Other Biological Sciences
dc.title Magnetic Resonance Imaging Correlates of White Matter Gliosis and Injury in Preterm Fetal Sheep Exposed to Progressive Systemic Inflammation.
dc.type Journal Article
dc.identifier.doi 10.3390/ijms21238891
pubs.issue 23
pubs.begin-page 8891
pubs.volume 21
dc.date.updated 2022-02-10T04:40:13Z
dc.rights.holder Copyright: The author en
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/33255257
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/OpenAccess en
pubs.subtype research-article
pubs.subtype Journal Article
pubs.elements-id 829685
dc.identifier.eissn 1422-0067
dc.identifier.pii ijms21238891
pubs.number ARTN 8891
pubs.online-publication-date 2020-11-24


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