The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABA<sub>A</sub> Receptor Subunit Expression in the Mouse SCN.

Show simple item record Chong, Janelle Cheeseman, James Frederick Pawley, Matthew DM Kwakowsky, Andrea Warman, Guy R
dc.coverage.spatial Switzerland 2022-04-21T04:21:46Z 2022-04-21T04:21:46Z 2021-9-17
dc.identifier.citation Clocks & sleep 3(3):482-494 17 Sep 2021
dc.identifier.issn 2624-5175
dc.description.abstract General anaesthesia (GA) is known to affect the circadian clock. However, the mechanisms that underlie GA-induced shifting of the clock are less well understood. Activation of γ-aminobutyric acid (GABA)<sub>-</sub>type A receptors (GABA<sub>A</sub>R) in the suprachiasmatic nucleus (SCN) can phase shift the clock and thus GABA and its receptors represent a putative pathway via which GA exerts its effect on the clock. Here, we investigated the concurrent effects of the inhalational anaesthetic, isoflurane, and light, on mouse behavioural locomotor rhythms and on α1, β3, and γ2 GABA<sub>A</sub>R subunit expression in the SCN of the mouse brain. Behavioural phase shifts elicited by exposure of mice to four hours of GA (2% isoflurane) and light (400 lux) (n = 60) were determined by recording running wheel activity rhythms in constant conditions (DD). Full phase response curves for the effects of GA + light on behavioural rhythms show that phase shifts persist in anaesthetized mice exposed to light. Daily variation was detected in all three GABA<sub>A</sub>R subunits in LD 12:12. The γ2 subunit expression was significantly increased following GA in DD (compared to light alone) at times of large behavioural phase delays. We conclude that the phase shifting effect of light on the mouse clock is not blocked by GA administration, and that γ2 may potentially be involved in the phase shifting effect of GA on the clock. Further analysis of GABA<sub>A</sub>R subunit expression in the SCN will be necessary to confirm its role.
dc.format.medium Electronic
dc.language eng
dc.publisher MDPI AG
dc.relation.ispartofseries Clocks & sleep
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject GABA
dc.subject GABAA receptor
dc.subject circadian clock
dc.subject general anaesthesia
dc.subject phase response curve
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Clinical Neurology
dc.subject Neurosciences
dc.subject Neurosciences & Neurology
dc.subject general anaesthesia
dc.subject circadian clock
dc.subject phase response curve
dc.subject GABA
dc.subject GABA(A) receptor
dc.subject SUBTYPES
dc.subject SHIFTS
dc.subject CLOCK
dc.title The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABA<sub>A</sub> Receptor Subunit Expression in the Mouse SCN.
dc.type Journal Article
dc.identifier.doi 10.3390/clockssleep3030034
pubs.issue 3
pubs.begin-page 482
pubs.volume 3 2022-03-07T21:16:42Z
dc.rights.holder Copyright: The author en
pubs.end-page 494
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype research-article
pubs.subtype Journal Article
pubs.elements-id 867336
dc.identifier.eissn 2624-5175
dc.identifier.pii clockssleep3030034 2021-9-17

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