Genes expressed during development of Pinus radiata male cones

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dc.contributor.advisor Gardner, Richard en Walden, Adrian R. en 2007-06-29 en 2007-06-29 en 1999 en
dc.identifier THESIS 00-328 en
dc.identifier.citation Thesis (PhD--Biological Sciences)--University of Auckland, 1999 en
dc.identifier.uri en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Differentially expressed genes involved in the flowering process have been isolated from various angiosperm species and functions for a few of them have been characterised. In contrast there is only a small amount of corresponding information available in conifers. Homology between regulatory genes involved in sexual reproduction in angiosperms and gymnosperms makes it likely that there are similar regulatory cascades controlling development of angiosperm flowers and gymnosperm cones. In order to engineer sterility in the conifer P. radiata it will be useful to identify P. radiata genes involved in the flowering process. This thesis describes the isolation and characterisation of cDNAs for six MADS-box transcription factors and 13 families of male-cone-specific cDNAs from P. radiata male cones. Sequence comparisons, phylogenetic analysis and spatial expression data indicate that the six MADS-box transcription factors from P. radiata are orthologues of the tomato gene TM3. TM3 has been isolated from several angiosperm species and does not appear to have a significant role in reproductive development. All of the TM3 homologues, including those in P. radiate, are expressed in vegetative as well as reproductive tissues. It is notable that there are at least six TM3 paralogues in P. radiata, two of which have deletions within the conserved K-domain. The temporal expression of thirteen male-cone-specific cDNA families during male cone development is described. All are expressed within the period between meiosis and the first microspore mitosis. In situ hybridisation analysis of five families is presented; four are expressed in the tapetum and one is expressed in the tetrad. Several of the cDNAs have striking similarity to anther-expressed genes from angiosperms and several are also homologues of defence and pathogen response genes. The potential functions of these pathogen/stress response genes in male cone development is discussed. In future these cDNAs could be used to isolate P. radiata promoters which will direct expression to the male cones. en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA9991357014002091 en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri en
dc.title Genes expressed during development of Pinus radiata male cones en
dc.type Thesis en Biological Sciences en The University of Auckland en Doctoral en PhD en
dc.rights.holder Copyright: The author en

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