Advancing respiratory-cardiovascular physiology with the working heart-brainstem preparation over 25 years.

Show simple item record Paton, Julian FR Machado, Benedito H Moraes, Davi JA Zoccal, Daniel B Abdala, Ana P Smith, Jeffrey C Antunes, Vagner R Murphy, David Dutschmann, Mathias Dhingra, Rishi R McAllen, Robin Pickering, Anthony E Wilson, Richard JA Day, Trevor A Barioni, Nicole O Allen, Andrew M Menuet, Clément Donnelly, Joseph Felippe, Igor St-John, Walter M
dc.coverage.spatial England 2022-05-06T03:47:25Z 2022-05-06T03:47:25Z 2022-03-16
dc.identifier.citation (2022). The Journal of Physiology.
dc.identifier.issn 0022-3751
dc.description.abstract Twenty-five years ago a new physiological preparation called the working heart-brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some progress made with the WHBP, some advantages and disadvantages, potential future applications and photographs/technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of the brain for whole cell recording and an uncompromised use of pharmacological agents akin to in vitro approaches. The preparation has revealed novel mechanistic insights into, for example, the generation of distinct respiratory rhythms, the neurogenesis of sympathetic activity, coupling between respiration and the heart and circulation, hypothalamic and spinal control mechanisms, peripheral and central chemoreceptor mechanisms . Insights have been gleaned into diseases such as hypertension, heart failure and sleep apnoea. Data from the in situ preparation have been ratified in conscious in vivo animals and when tested has translated to humans. We conclude by discussing potential future applications of the WHBP including two-photon imaging of peripheral and central nervous systems and adoption of pharmacogenetic tools that will improve our understanding of physiological mechanisms and reveal novel mechanisms that may guide new treatment strategies for cardiorespiratory diseases. Abstract figure legend The working heart brainstem preparation (WHBP) was first published in 1996 and since that time has revealed novel mechanisms in the generation of breathing rhythms and patterns, autonomic control of the heart and circulation, and the physiological relevance of the coupling between respiratory and cardiovascular systems. This review brings together an international cohort of authors that adopted the WHBP to highlight some of the major advances and discoveries made using this in situ arterially perfused preparation. This article is protected by copyright. All rights reserved.
dc.format.medium Print-Electronic
dc.language eng
dc.publisher Wiley
dc.relation.ispartofseries The Journal of physiology
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Kolliker Fuse
dc.subject cardiac ganglion
dc.subject cardiac vagus
dc.subject chemoreceptor
dc.subject eupnoea
dc.subject gasp
dc.subject hypertension
dc.subject hypoxia
dc.subject pre-Bötzinger complex
dc.subject respiratory rhythm generation
dc.subject sympathetic
dc.subject sympathetic-respiratory coupling
dc.subject Heart Disease
dc.subject Lung
dc.subject Neurosciences
dc.subject Cardiovascular
dc.subject 06 Biological Sciences
dc.subject 11 Medical and Health Sciences
dc.title Advancing respiratory-cardiovascular physiology with the working heart-brainstem preparation over 25 years.
dc.type Journal Article
dc.identifier.doi 10.1113/jp281953 2022-04-04T21:27:04Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 35294064 (pubmed)
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Journal Article
pubs.elements-id 889605 Medical and Health Sciences Medical Sciences Physiology Division
dc.identifier.eissn 1469-7793
pubs.record-created-at-source-date 2022-04-05 2022-03-16

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