Variable colocalisation of GABAA receptor subunits and glycine receptors on neurons in the human hypoglossal nucleus.

Show simple item record Waldvogel, HJ Biggins, FM Singh, A Arasaratnam, CJ Faull, RLM
dc.coverage.spatial Netherlands 2022-05-19T04:55:46Z 2022-05-19T04:55:46Z 2019-04
dc.identifier.citation (2019). Journal of Chemical Neuroanatomy, 97, 99-111.
dc.identifier.issn 0891-0618
dc.description.abstract The hypoglossal nucleus, the nucleus of the twelfth cranial nerve, is located dorsally in the midline of the medulla oblongata. The hypoglossal nucleus contains lower motor neurons which innervate the tongue muscles that control tongue movements involved in speech production, swallowing, mastication and associated respiratory movements. GABAA and glycine receptors are heteropentameric ionotropic receptors that facilitate fast-response, inhibitory neurotransmission in the mammalian brain and spinal cord. We investigated the immunohistochemical distribution of the GABAA receptor α1, α2, β2,3 subunits and glycine receptors as well as their relationship to the vesicular GABA transporter (VGAT) in the human hypoglossal nucleus at the light and confocal laser scanning microscope levels. The results showed that all of the GABAA receptor subunits as well as glycine receptor display punctate labelling indicative of synapses on the soma and dendritic membranes of large neurons within the hypoglossal nucleus. On average, approximately 50% of glycine receptors were co localised with GABAA receptor  α1 subunits. Also on average GABAA α2 and β2,3 subunits were colocalised with approximately 30% of glycine receptor subunits. VGAT positive terminals were associated with both GABAA and glycine receptor types. Both glycinergic and GABAergic positive puncta were found adjacent to VGAT terminal-like staining. These results suggest that inhibition of human hypoglossal motor neurons occurs not only through complex interaction of separated GABAAR and glycine receptor regions, but also through synapses containing both inhibitory receptor types co-existing at the same synaptic sites.
dc.format.medium Print-Electronic
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartofseries Journal of chemical neuroanatomy
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Medulla Oblongata
dc.subject Neurons
dc.subject Motor Neurons
dc.subject Hypoglossal Nerve
dc.subject Humans
dc.subject Receptors, GABA-A
dc.subject Receptors, Glycine
dc.subject Adult
dc.subject Aged
dc.subject Aged, 80 and over
dc.subject Middle Aged
dc.subject Female
dc.subject Male
dc.subject Antibodies
dc.subject Confocal
dc.subject GABA
dc.subject Glycine
dc.subject Human brain
dc.subject Hypoglossal nucleus
dc.subject Immunohistochemistry
dc.subject Microscopy
dc.subject Receptors
dc.subject Subunits
dc.subject XII cranial nerve nucleus
dc.subject Neurosciences
dc.subject Neurological
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Biochemistry & Molecular Biology
dc.subject Neurosciences & Neurology
dc.subject MOTOR NUCLEUS
dc.subject SPINAL-CORD
dc.subject A-RECEPTOR
dc.subject ALPHA-1 SUBUNIT
dc.subject BETA-SUBUNIT
dc.subject 1109 Neurosciences
dc.subject Clinical
dc.subject Basic Science
dc.subject Dental/Oral and Craniofacial Disease
dc.title Variable colocalisation of GABAA receptor subunits and glycine receptors on neurons in the human hypoglossal nucleus.
dc.type Journal Article
dc.identifier.doi 10.1016/j.jchemneu.2019.02.005
pubs.begin-page 99
pubs.volume 97 2022-04-10T23:47:32Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 30825508 (pubmed)
pubs.end-page 111
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Research Support, Non-U.S. Gov't
pubs.subtype Journal Article
pubs.elements-id 766090 Medical and Health Sciences Medical Sciences Anatomy and Medical Imaging
dc.identifier.eissn 1873-6300
dc.identifier.pii S0891-0618(18)30059-0
pubs.record-created-at-source-date 2022-04-11

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