Detecting Phytoplankton Cell Viability Using NIR Raman Spectroscopy and PCA.

Show simple item record Novikova, Nina I Matthews, Hannah Williams, Isabelle Sewell, Mary A Nieuwoudt, Michel K Simpson, M Cather Broderick, Neil GR
dc.coverage.spatial United States 2022-06-10T04:27:04Z 2022-06-10T04:27:04Z 2022-02-10
dc.identifier.citation (2022). ACS Omega, 7(7), 5962-5971.
dc.identifier.issn 2470-1343
dc.description.abstract Raman spectroscopy has long been suggested as a potentially fast and sensitive method to monitor phytoplankton abundance and composition in marine environments. However, the pitfalls of visible detection methods in pigment-rich biological material and the complexity of their spectra have hindered their application as reliable <i>in situ</i> detection methods. In this study we combine 1064 nm confocal Raman spectroscopy with multivariate statistical analysis techniques (principle component analysis and partial leas-squares discriminant analysis) to reliably measure differences in the cell viability of a diatom species (<i>Chaetoceros muelleri</i>) and two haptophyte species (<i>Diacronema lutheri</i> and <i>Tisochrysis lutea</i>) of phytoplankton. The low fluorescence background due to this combined approach of NIR Raman spectroscopy and multivariate data analysis allowed small changes in the overall spectral profiles to be reliably monitored, enabling the identification of the specific spectral features that could classify cells as viable or nonviable regardless of their species. The most significant differences upon cell death were shown by characteristic shifts in the carotenoid bands at 1527 and 1158 cm<sup>-1</sup>. The contributions from other biomolecules were less pronounced but revealed changes that could be identified using this combination of techniques.
dc.format.medium Electronic-eCollection
dc.language eng
dc.publisher American Chemical Society (ACS)
dc.relation.ispartofseries ACS omega
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject 4.1 Discovery and preclinical testing of markers and technologies
dc.subject Science & Technology
dc.subject Physical Sciences
dc.subject Chemistry, Multidisciplinary
dc.subject Chemistry
dc.subject SPECTRA
dc.subject OCEAN
dc.subject 0904 Chemical Engineering
dc.subject 0912 Materials Engineering
dc.title Detecting Phytoplankton Cell Viability Using NIR Raman Spectroscopy and PCA.
dc.type Journal Article
dc.identifier.doi 10.1021/acsomega.1c06262
pubs.issue 7
pubs.begin-page 5962
pubs.volume 7 2022-05-16T05:38:34Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 35224357 (pubmed)
pubs.end-page 5971
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype research-article
pubs.subtype Journal Article
pubs.elements-id 882703 Science Chemistry Physics Biological Sciences
dc.identifier.eissn 2470-1343
pubs.record-created-at-source-date 2022-05-16 2022-02-10

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