Skin swabbing of amphibian larvae yields sufficient DNA for efficient sequencing and reliable microsatellite genotyping

Show simple item record Pichlmüller, Florian Straub, Christina Helfer, Véronique 2022-06-13T00:00:42Z 2022-06-13T00:00:42Z 2013-01-01
dc.identifier.citation (2013). Amphibia-Reptilia, 34(4), 517-523.
dc.identifier.issn 0173-5373
dc.description.abstract <jats:p>Skin swabbing, a minimally invasive DNA sampling method recently developed on adult amphibians, was tested on larvae of fire salamanders (<jats:italic>Salamandra salamandra</jats:italic>). The quality and quantity of the sampled DNA was evaluated by (i) measuring DNA concentration in DNA extracts, (ii) sequencing part of the <jats:italic>mt</jats:italic>DNA cytochrome <jats:italic>b</jats:italic> gene (692 bp) and (iii) genotyping eight polymorphic nuclear microsatellite loci. The multiple-tubes approach was used for calculating allelic dropout (ADO) and false allele (FA) rates to evaluate the reliability of the genotypes. DNA extracts from tissue samples of road-killed individuals were included in the study as positive controls. Our results showed that skin swabs of fire salamander larvae can provide DNA in sufficient quantity and quality, as sequencing was successful and no allelic dropouts or false alleles were detected. This method, tested for the first time on amphibian larvae, has proven to be an efficient and reliable alternative to the controversial tail fin clipping procedure.</jats:p>
dc.language English
dc.publisher Brill
dc.relation.ispartofseries Amphibia-Reptilia
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Genetics
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Zoology
dc.subject allelic dropouts
dc.subject false alleles
dc.subject fire salamanders
dc.subject minimally invasive sampling
dc.subject Salamandra salamandra
dc.subject BUCCAL SWABS
dc.subject SAMPLES
dc.subject PCR
dc.subject 0608 Zoology
dc.title Skin swabbing of amphibian larvae yields sufficient DNA for efficient sequencing and reliable microsatellite genotyping
dc.type Journal Article
dc.identifier.doi 10.1163/15685381-00002909
pubs.issue 4
pubs.begin-page 517
pubs.volume 34 2022-05-10T02:12:01Z
dc.rights.holder Copyright: The author en
pubs.end-page 523
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Article
pubs.subtype Journal
pubs.elements-id 501771 Science Biological Sciences
dc.identifier.eissn 0173-5373
pubs.record-created-at-source-date 2022-05-10

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