dc.contributor.author |
Heller, M |
|
dc.contributor.author |
Loomes, KM |
|
dc.contributor.author |
Cooper, GJ |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2022-06-13T03:04:54Z |
|
dc.date.available |
2022-06-13T03:04:54Z |
|
dc.date.issued |
2000-10 |
|
dc.identifier.citation |
(2000). Analytical Biochemistry, 285(1), 100-104. |
|
dc.identifier.issn |
0003-2697 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/59777 |
|
dc.description.abstract |
Amylin is a hormone belonging to the calcitonin protein family of peptides. To facilitate receptor screening studies, alternatively radiolabeled and biologically active amylin and salmon calcitonin analogues were synthesized by reductive methylation. Free amino groups of amylin and salmon calcitonin were methylated by reaction of peptides with formaldehyde and sodium [(3)H]borohydride. Radioactively labeled peptides were purified by size exclusion chromatography followed by HPLC. Analysis by MALDI-TOF mass spectrometry of purified amylin and salmon calcitonin peptides revealed incorporation of both two and four tritiated methyl groups per peptide molecule. Specific activities of 22.6 and 23.2 GBq/mmol were measured for amylin and salmon calcitonin, respectively. Methylation of rat amylin and salmon calcitonin did not affect their biological activities as both retained their potency to inhibit insulin-stimulated glycogen synthesis in isolated rat soleus muscle. The synthesis of these tritiated analogues provides an alternative chemically stable radiolabeled ligand which may be useful in exploring receptor interactions within the calcitonin peptide family. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Elsevier BV |
|
dc.relation.ispartofseries |
Analytical biochemistry |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Muscle, Skeletal |
|
dc.subject |
Animals |
|
dc.subject |
Rats |
|
dc.subject |
Rats, Wistar |
|
dc.subject |
Tritium |
|
dc.subject |
Carbon Radioisotopes |
|
dc.subject |
Amyloid |
|
dc.subject |
Glycogen |
|
dc.subject |
Calcitonin |
|
dc.subject |
Chromatography, High Pressure Liquid |
|
dc.subject |
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
|
dc.subject |
Male |
|
dc.subject |
Islet Amyloid Polypeptide |
|
dc.subject |
In Vitro Techniques |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Physical Sciences |
|
dc.subject |
Biochemical Research Methods |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Chemistry, Analytical |
|
dc.subject |
Chemistry |
|
dc.subject |
amylin |
|
dc.subject |
type 2 diabetes |
|
dc.subject |
receptor binding |
|
dc.subject |
insulin resistance |
|
dc.subject |
GENE-RELATED PEPTIDE |
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dc.subject |
BINDING-SITES |
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dc.subject |
RECEPTORS |
|
dc.subject |
CGRP |
|
dc.subject |
PROTEIN |
|
dc.subject |
LUNG |
|
dc.subject |
IAPP |
|
dc.subject |
0305 Organic Chemistry |
|
dc.subject |
0301 Analytical Chemistry |
|
dc.subject |
0399 Other Chemical Sciences |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.title |
Synthesis of biologically active tritiated amylin and salmon calcitonin analogues. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1006/abio.2000.4716 |
|
pubs.issue |
1 |
|
pubs.begin-page |
100 |
|
pubs.volume |
285 |
|
dc.date.updated |
2022-05-03T04:58:27Z |
|
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
10998268 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/10998268 |
|
pubs.end-page |
104 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Journal Article |
|
pubs.elements-id |
3006 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1096-0309 |
|
dc.identifier.pii |
S0003-2697(00)94716-X |
|
pubs.record-created-at-source-date |
2022-05-03 |
|