The natural compound rutaecarpine promotes white adipocyte browning through activation of the AMPK-PRDM16 axis.

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dc.contributor.author Liu, Xiaomin
dc.contributor.author Zhang, Yuwei
dc.contributor.author Chu, Yi
dc.contributor.author Zhao, Xuemei
dc.contributor.author Mao, Liufeng
dc.contributor.author Zhao, Shiting
dc.contributor.author Lin, Shaoqiang
dc.contributor.author Hui, Xiaoyan
dc.contributor.author Gu, Ping
dc.contributor.author Xu, Yong
dc.contributor.author Loomes, Kerry
dc.contributor.author Tang, Shibing
dc.contributor.author Nie, Tao
dc.contributor.author Wu, Donghai
dc.coverage.spatial United States
dc.date.accessioned 2022-06-13T04:05:37Z
dc.date.available 2022-06-13T04:05:37Z
dc.date.issued 2021-03
dc.identifier.citation (2021). Biochemical and Biophysical Research Communications, 545, 189-194.
dc.identifier.issn 0006-291X
dc.identifier.uri https://hdl.handle.net/2292/59785
dc.description.abstract The prevalence of obesity is increasing globally and is associated with many metabolic disorders, such as type 2 diabetes and cardiovascular diseases. In recent years, a number of studies suggest that promotion of white adipose browning represents a promising strategy to combat obesity and its related metabolic disorders. The aim of this study was to identify compounds that induce adipocyte browning and elucidate their mechanism of action. Among the 500 natural compounds screened, a small molecule named Rutaecarpine, was identified as a positive regulator of adipocyte browning both in vitro and in vivo. KEGG pathway analysis from RNA-seq data suggested that the AMPK signaling pathway was regulated by Rutaecarpine, which was validated by Western blot analysis. Furthermore, inhibition of AMPK signaling mitigated the browning effect of Rutaecaripine. The effect of Rutaecaripine on adipocyte browning was also abolished upon deletion of Prdm16, a downstream target of AMPK pathway. In collusion, Rutaecarpine is a potent chemical agent to induce adipocyte browning and may serve as a potential drug candidate to treat obesity.
dc.format.medium Print-Electronic
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartofseries Biochemical and biophysical research communications
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject Animals
dc.subject Mice, Transgenic
dc.subject Mice
dc.subject Obesity
dc.subject Disease Models, Animal
dc.subject Indole Alkaloids
dc.subject Quinazolines
dc.subject DNA-Binding Proteins
dc.subject Transcription Factors
dc.subject Biological Products
dc.subject Drug Evaluation, Preclinical
dc.subject Signal Transduction
dc.subject Oxygen Consumption
dc.subject Thermogenesis
dc.subject Models, Biological
dc.subject Male
dc.subject Adipocytes, White
dc.subject AMP-Activated Protein Kinases
dc.subject In Vitro Techniques
dc.subject Adipocytes, Beige
dc.subject AMPK
dc.subject Beige adipocyte
dc.subject Browning
dc.subject Rutaecarpine
dc.subject White adipocyte
dc.subject Diabetes
dc.subject Nutrition
dc.subject 5.1 Pharmaceuticals
dc.subject Metabolic and endocrine
dc.subject 0304 Medicinal and Biomolecular Chemistry
dc.subject 0601 Biochemistry and Cell Biology
dc.subject 1101 Medical Biochemistry and Metabolomics
dc.title The natural compound rutaecarpine promotes white adipocyte browning through activation of the AMPK-PRDM16 axis.
dc.type Journal Article
dc.identifier.doi 10.1016/j.bbrc.2021.01.080
pubs.begin-page 189
pubs.volume 545
dc.date.updated 2022-05-02T06:16:16Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 33561654 (pubmed)
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/33561654
pubs.end-page 194
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/RetrictedAccess en
pubs.subtype Research Support, Non-U.S. Gov't
pubs.subtype Journal Article
pubs.elements-id 838614
pubs.org-id Science
pubs.org-id Biological Sciences
pubs.org-id Science Research
pubs.org-id Maurice Wilkins Centre (2010-2014)
dc.identifier.eissn 1090-2104
dc.identifier.pii S0006-291X(21)00139-X
pubs.record-created-at-source-date 2022-05-02


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