Proteomic analysis of honey. Identification of unique peptide markers for authentication of NZ mānuka (Leptospermum scoparium) honey.

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dc.contributor.author Bong, Jessie
dc.contributor.author Middleditch, Martin
dc.contributor.author Loomes, Kerry M
dc.contributor.author Stephens, Jonathan M
dc.coverage.spatial England
dc.date.accessioned 2022-06-13T22:23:57Z
dc.date.available 2022-06-13T22:23:57Z
dc.date.issued 2021-07
dc.identifier.citation (2021). Food Chemistry, 350, 128442-.
dc.identifier.issn 0308-8146
dc.identifier.uri https://hdl.handle.net/2292/59801
dc.description.abstract Proteomics is an emerging tool in food authentication that has not been optimised for honey analysis. In this study, we present a qualitative proteomic analysis of New Zealand mānuka (Leptospermum scoparium) honey. A total of fifty bee-derived proteins were identified in the honey, the most predominant being major royal jelly proteins (MRJPs). We also demonstrate for the first time the presence of unique nectar-derived proteins in mānuka honey. A total of 17 mānuka plant proteins were identified, a-third of which were putative pathogenesis-related proteins. Two proteins involved in drought tolerance were also identified. Twelve candidate peptides were selected as potential authentication markers based on their uniqueness to mānuka honey. Nectar analyses confirmed the origin and specificity of these peptides to L. scoparium nectar, thus presenting peptide profiling as a viable and novel approach for mānuka honey authentication. Raw data are available via ProteomeXchange with identifier PXD021730.
dc.format.medium Print-Electronic
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartofseries Food chemistry
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject Leptospermum
dc.subject Peptides
dc.subject Proteomics
dc.subject Plant Nectar
dc.subject Biomarkers
dc.subject Leptospermum scoparium
dc.subject Mānuka
dc.subject Nectar
dc.subject Peptide
dc.subject Science & Technology
dc.subject Physical Sciences
dc.subject Life Sciences & Biomedicine
dc.subject Chemistry, Applied
dc.subject Food Science & Technology
dc.subject Nutrition & Dietetics
dc.subject Chemistry
dc.subject Ma?nuka
dc.title Proteomic analysis of honey. Identification of unique peptide markers for authentication of NZ mānuka (Leptospermum scoparium) honey.
dc.type Journal Article
dc.identifier.doi 10.1016/j.foodchem.2020.128442
pubs.begin-page 128442
pubs.volume 350
dc.date.updated 2022-05-02T06:00:25Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 33388180 (pubmed)
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/33388180
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/RetrictedAccess en
pubs.subtype Journal Article
pubs.elements-id 823225
pubs.org-id Science
pubs.org-id Biological Sciences
pubs.org-id Science Research
pubs.org-id Maurice Wilkins Centre (2010-2014)
dc.identifier.eissn 1873-7072
dc.identifier.pii S0308-8146(20)32304-9
pubs.number 128442
pubs.record-created-at-source-date 2022-05-02


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