dc.contributor.author |
Bunker, Richard D |
|
dc.contributor.author |
Loomes, Kerry M |
|
dc.contributor.author |
Baker, Edward N |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2022-06-16T02:29:42Z |
|
dc.date.available |
2022-06-16T02:29:42Z |
|
dc.date.issued |
2012-01 |
|
dc.identifier.citation |
(2012). Acta Crystallographica. Section F: Structural Biology Communications, 68(Pt 1), 59-62. |
|
dc.identifier.issn |
2053-230X |
|
dc.identifier.uri |
https://hdl.handle.net/2292/59920 |
|
dc.description.abstract |
Human dihydrodipicolinate synthase-like protein (DHDPSL) is a gene product of unknown function. It is homologous to bacterial pyruvate-dependent aldolases such as dihydrodipicolinate synthase (DHDPS), which functions in lysine biosynthesis. However, it cannot have this function and instead is implicated in a genetic disorder that leads to excessive production of oxalate and kidney-stone formation. In order to better understand its function, DHDPSL was expressed as an MBP-fusion protein and crystallized using an in situ proteolysis protocol. Two crystal forms were obtained, both of which diffracted X-rays to approximately 2.0 Å resolution. One of these, belonging to space group P6(2)22 or P6(4)22 with unit-cell parameters a = b = 142.9, c = 109.8 Å, α = β = 90, γ = 120°, was highly reproducible and suitable for structure determination by X-ray crystallography. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
International Union of Crystallography (IUCr) |
|
dc.relation.ispartofseries |
Acta crystallographica. Section F, Structural biology and crystallization communications |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://journals.iucr.org/services/copyrightpolicy.html |
|
dc.subject |
Humans |
|
dc.subject |
Hydro-Lyases |
|
dc.subject |
Crystallization |
|
dc.subject |
Crystallography, X-Ray |
|
dc.subject |
Urologic Diseases |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Physical Sciences |
|
dc.subject |
Biochemical Research Methods |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Biophysics |
|
dc.subject |
Crystallography |
|
dc.subject |
primary oxaluria |
|
dc.subject |
DHDPS |
|
dc.subject |
aldolase |
|
dc.subject |
N-ACETYLNEURAMINATE LYASE |
|
dc.subject |
ESCHERICHIA-COLI |
|
dc.subject |
SUBFAMILY |
|
dc.subject |
CRYSTALS |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.subject |
Biomedical |
|
dc.subject |
Basic Science |
|
dc.subject |
03 Chemical Sciences |
|
dc.subject |
06 Biological Sciences |
|
dc.title |
Purification, crystallization and preliminary crystallographic analysis of human dihydrodipicolinate synthase-like protein (DHDPSL). |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1107/s1744309111048068 |
|
pubs.issue |
Pt 1 |
|
pubs.begin-page |
59 |
|
pubs.volume |
68 |
|
dc.date.updated |
2022-05-03T04:22:38Z |
|
dc.rights.holder |
Copyright: International Union of Crystallography |
en |
dc.identifier.pmid |
22232173 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/22232173 |
|
pubs.end-page |
62 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
277072 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1744-3091 |
|
dc.identifier.pii |
S1744309111048068 |
|
pubs.record-created-at-source-date |
2022-05-03 |
|
pubs.online-publication-date |
2011-12-24 |
|