The isolated perfused rat kidney: a technical update

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dc.contributor.author Chang, H
dc.contributor.author Choong
dc.contributor.author Phillips, A
dc.contributor.author Loomes, KM
dc.coverage.spatial Japan
dc.date.accessioned 2022-06-16T03:28:53Z
dc.date.available 2022-06-16T03:28:53Z
dc.date.issued 2013
dc.identifier.citation (2013). Experimental Animals, 62(1), 19-23.
dc.identifier.issn 1341-1357
dc.identifier.uri https://hdl.handle.net/2292/59930
dc.description.abstract The isolated perfused kidney is commonly utilized as a screening tool for renal clearance and metabolism, and to correlate renal drug deposition to renal function. Here, we report on several aspects of this procedure that will facilitate a higher experimental success rate and lead to a reduction in animal use. First, we investigated the utility of inulin and creatinine as commonly used markers to measure glomerular filtration rate. For inulin, in the presence of either 20 mM glucose or 4.5% dextran in the buffer, significant interference was observed using an anthrone-based colorimetric assay. These findings suggest that caution needs to be exercised when using glucose or dextran and when inulin is quantitated using this method. Under these circumstances the use of alternative methods of inulin quantitation such as fluorescently tagged inulin is preferred. Second, we optimized bovine serum albumin (BSA) and BSA/dextran compositions that are routinely recommended as oncotic agents in the perfusion buffer and found that a 4% BSA/1.67% dextran composition had the best viability of kidney biomarkers in accordance with recommended threshold parameters. These considerations will be of particular relevance to researchers utilizing the isolated perfused kidney as a screening tool to measure renal biology and drug metabolism as well as applications to investigate diabetic nephropathy.
dc.format.medium Print
dc.language eng
dc.publisher Japanese Association for Laboratory Animal Science
dc.relation.ispartofseries Experimental Animals
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject Kidney
dc.subject Animals
dc.subject Rats
dc.subject Rats, Wistar
dc.subject Diabetic Nephropathies
dc.subject Creatinine
dc.subject Dextrans
dc.subject Insulin
dc.subject Glucose
dc.subject Serum Albumin, Bovine
dc.subject Glomerular Filtration Rate
dc.subject Perfusion
dc.subject Tissue Survival
dc.subject Male
dc.subject In Vitro Techniques
dc.subject Kidney Disease
dc.subject Diabetes
dc.subject Renal and urogenital
dc.subject Science & Technology
dc.subject Life Sciences & Biomedicine
dc.subject Veterinary Sciences
dc.subject Zoology
dc.subject diabetic nephropathy
dc.subject isolated perfused kidney
dc.subject 1103 Clinical Sciences
dc.subject Clinical Medicine and Science
dc.subject 06 Biological Sciences
dc.subject 07 Agricultural and Veterinary Sciences
dc.subject 11 Medical and Health Sciences
dc.title The isolated perfused rat kidney: a technical update
dc.type Journal Article
dc.identifier.doi 10.1538/expanim.62.19
pubs.issue 1
pubs.begin-page 19
pubs.volume 62
dc.date.updated 2022-05-02T09:05:04Z
dc.rights.holder Copyright: The author en
dc.identifier.pmid 23357942 (pubmed)
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/23357942
pubs.end-page 23
pubs.publication-status Published
dc.rights.accessrights http://purl.org/eprint/accessRights/RetrictedAccess en
pubs.subtype Article
pubs.elements-id 365268
pubs.org-id Medical and Health Sciences
pubs.org-id Science
pubs.org-id Biological Sciences
pubs.org-id Science Research
pubs.org-id Medical Sciences
pubs.org-id Molecular Medicine
pubs.org-id Maurice Wilkins Centre (2010-2014)
dc.identifier.eissn 1881-7122
dc.identifier.pii DN/JST.JSTAGE/expanim/62.19
pubs.record-created-at-source-date 2022-05-02
pubs.online-publication-date 2013-01-25


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