dc.contributor.author |
Chang, H |
|
dc.contributor.author |
Choong |
|
dc.contributor.author |
Phillips, A |
|
dc.contributor.author |
Loomes, KM |
|
dc.coverage.spatial |
Japan |
|
dc.date.accessioned |
2022-06-16T03:28:53Z |
|
dc.date.available |
2022-06-16T03:28:53Z |
|
dc.date.issued |
2013 |
|
dc.identifier.citation |
(2013). Experimental Animals, 62(1), 19-23. |
|
dc.identifier.issn |
1341-1357 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/59930 |
|
dc.description.abstract |
The isolated perfused kidney is commonly utilized as a screening tool for renal clearance and metabolism, and to correlate renal drug deposition to renal function. Here, we report on several aspects of this procedure that will facilitate a higher experimental success rate and lead to a reduction in animal use. First, we investigated the utility of inulin and creatinine as commonly used markers to measure glomerular filtration rate. For inulin, in the presence of either 20 mM glucose or 4.5% dextran in the buffer, significant interference was observed using an anthrone-based colorimetric assay. These findings suggest that caution needs to be exercised when using glucose or dextran and when inulin is quantitated using this method. Under these circumstances the use of alternative methods of inulin quantitation such as fluorescently tagged inulin is preferred. Second, we optimized bovine serum albumin (BSA) and BSA/dextran compositions that are routinely recommended as oncotic agents in the perfusion buffer and found that a 4% BSA/1.67% dextran composition had the best viability of kidney biomarkers in accordance with recommended threshold parameters. These considerations will be of particular relevance to researchers utilizing the isolated perfused kidney as a screening tool to measure renal biology and drug metabolism as well as applications to investigate diabetic nephropathy. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Japanese Association for Laboratory Animal Science |
|
dc.relation.ispartofseries |
Experimental Animals |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Kidney |
|
dc.subject |
Animals |
|
dc.subject |
Rats |
|
dc.subject |
Rats, Wistar |
|
dc.subject |
Diabetic Nephropathies |
|
dc.subject |
Creatinine |
|
dc.subject |
Dextrans |
|
dc.subject |
Insulin |
|
dc.subject |
Glucose |
|
dc.subject |
Serum Albumin, Bovine |
|
dc.subject |
Glomerular Filtration Rate |
|
dc.subject |
Perfusion |
|
dc.subject |
Tissue Survival |
|
dc.subject |
Male |
|
dc.subject |
In Vitro Techniques |
|
dc.subject |
Kidney Disease |
|
dc.subject |
Diabetes |
|
dc.subject |
Renal and urogenital |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Veterinary Sciences |
|
dc.subject |
Zoology |
|
dc.subject |
diabetic nephropathy |
|
dc.subject |
isolated perfused kidney |
|
dc.subject |
1103 Clinical Sciences |
|
dc.subject |
Clinical Medicine and Science |
|
dc.subject |
06 Biological Sciences |
|
dc.subject |
07 Agricultural and Veterinary Sciences |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
The isolated perfused rat kidney: a technical update |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1538/expanim.62.19 |
|
pubs.issue |
1 |
|
pubs.begin-page |
19 |
|
pubs.volume |
62 |
|
dc.date.updated |
2022-05-02T09:05:04Z |
|
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
23357942 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/23357942 |
|
pubs.end-page |
23 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
|
pubs.elements-id |
365268 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
Medical Sciences |
|
pubs.org-id |
Molecular Medicine |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1881-7122 |
|
dc.identifier.pii |
DN/JST.JSTAGE/expanim/62.19 |
|
pubs.record-created-at-source-date |
2022-05-02 |
|
pubs.online-publication-date |
2013-01-25 |
|