dc.contributor.author |
Loomes, KM |
|
dc.contributor.author |
Senior, HE |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2022-06-23T04:35:35Z |
|
dc.date.available |
2022-06-23T04:35:35Z |
|
dc.date.issued |
1997-04 |
|
dc.identifier.citation |
(1997). FEBS Letters, 405(3), 369-372. |
|
dc.identifier.issn |
0014-5793 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/60120 |
|
dc.description.abstract |
Human milk cholesterol esterase (bile salt-activated lipase) plays a role in the dietary uptake of triacylglyceride and cholesteryl ester. The activities toward these substrates are mediated through a unique bile salt-activated mechanism. Previously, it has been proposed that a necessary step in this process is prior protein dimerisation in the presence of primary bile salts. In this study, we addressed the role of protein dimerisation by investigating bile salt interactions on full length and truncated recombinant forms, as analysed by size exclusion chromatography and concanavalin A Sepharose binding experiments. The present findings demonstrate that protein dimerisation is not an obligatory component of the bile salt-activated pathway. A new functional role for the glycosylated C-terminal domain in cholesterol esterase is also demonstrated in the prevention of non-specific hydrophobic interactions. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Wiley |
|
dc.relation.ispartofseries |
FEBS letters |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Humans |
|
dc.subject |
Salts |
|
dc.subject |
Bile Acids and Salts |
|
dc.subject |
Macromolecular Substances |
|
dc.subject |
Glycoproteins |
|
dc.subject |
Recombinant Proteins |
|
dc.subject |
Enzyme Activation |
|
dc.subject |
Protein Binding |
|
dc.subject |
Structure-Activity Relationship |
|
dc.subject |
Sterol Esterase |
|
dc.subject |
Nutrition |
|
dc.subject |
Digestive Diseases |
|
dc.subject |
Prevention |
|
dc.subject |
2.1 Biological and endogenous factors |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Biophysics |
|
dc.subject |
Cell Biology |
|
dc.subject |
cholesterol esterase |
|
dc.subject |
lysophospholipase |
|
dc.subject |
carboxylester lipase |
|
dc.subject |
bile salt activation |
|
dc.subject |
bile salt-stimulated lipase |
|
dc.subject |
PROLINE-RICH REPEATS |
|
dc.subject |
HUMAN-MILK |
|
dc.subject |
STIMULATED LIPASE |
|
dc.subject |
CANDIDA-CYLINDRACEA |
|
dc.subject |
INTERFACIAL ACTIVATION |
|
dc.subject |
HYDROLASE |
|
dc.subject |
GLYCOSYLATION |
|
dc.subject |
CONFORMATION |
|
dc.subject |
SUBSTRATE |
|
dc.subject |
IDENTITY |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.subject |
Biomedical |
|
dc.subject |
0304 Medicinal and Biomolecular Chemistry |
|
dc.subject |
0603 Evolutionary Biology |
|
dc.title |
Bile salt activation of human cholesterol esterase does not require protein dimerisation. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1016/s0014-5793(97)00215-9 |
|
pubs.issue |
3 |
|
pubs.begin-page |
369 |
|
pubs.volume |
405 |
|
dc.date.updated |
2022-05-05T01:44:52Z |
|
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
9108320 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/9108320 |
|
pubs.end-page |
372 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
45524 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1873-3468 |
|
dc.identifier.pii |
S0014-5793(97)00215-9 |
|
pubs.record-created-at-source-date |
2022-05-05 |
|
pubs.online-publication-date |
1997-11-07 |
|