dc.contributor.author |
Hemara, Lauren M |
|
dc.contributor.author |
Jayaraman, Jay |
|
dc.contributor.author |
Sutherland, Paul W |
|
dc.contributor.author |
Montefiori, Mirco |
|
dc.contributor.author |
Arshed, Saadiah |
|
dc.contributor.author |
Chatterjee, Abhishek |
|
dc.contributor.author |
Chen, Ronan |
|
dc.contributor.author |
Andersen, Mark T |
|
dc.contributor.author |
Mesarich, Carl H |
|
dc.contributor.author |
van der Linden, Otto |
|
dc.contributor.author |
Yoon, Minsoo |
|
dc.contributor.author |
Schipper, Magan M |
|
dc.contributor.author |
Vanneste, Joel L |
|
dc.contributor.author |
Brendolise, Cyril |
|
dc.contributor.author |
Templeton, Matthew D |
|
dc.contributor.editor |
Coaker, Gitta |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2022-07-26T00:09:54Z |
|
dc.date.available |
2022-07-26T00:09:54Z |
|
dc.date.issued |
2022-05-27 |
|
dc.identifier.citation |
(2022). PLoS Pathogens, 18(5), e1010542-. |
|
dc.identifier.issn |
1553-7366 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/60523 |
|
dc.description.abstract |
A pandemic isolate of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has devastated kiwifruit orchards growing cultivars of Actinidia chinensis. In contrast, A. arguta (kiwiberry) is not a host of Psa3. Resistance is mediated via effector-triggered immunity, as demonstrated by induction of the hypersensitive response in infected A. arguta leaves, observed by microscopy and quantified by ion-leakage assays. Isolates of Psa3 that cause disease in A. arguta have been isolated and analyzed, revealing a 51 kb deletion in the exchangeable effector locus (EEL). This natural EEL-mutant isolate and strains with synthetic knockouts of the EEL were more virulent in A. arguta plantlets than wild-type Psa3. Screening of a complete library of Psa3 effector knockout strains identified increased growth in planta for knockouts of four effectors-AvrRpm1a, HopF1c, HopZ5a, and the EEL effector HopAW1a -suggesting a resistance response in A. arguta. Hypersensitive response (HR) assays indicate that three of these effectors trigger a host species-specific HR. A Psa3 strain with all four effectors knocked out escaped host recognition, but a cumulative increase in bacterial pathogenicity and virulence was not observed. These avirulence effectors can be used in turn to identify the first cognate resistance genes in Actinidia for breeding durable resistance into future kiwifruit cultivars. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Public Library of Science (PLoS) |
|
dc.relation.ispartofseries |
PLoS pathogens |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Genetics |
|
dc.subject |
Vaccine Related |
|
dc.subject |
2.2 Factors relating to the physical environment |
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dc.subject |
2 Aetiology |
|
dc.subject |
Infection |
|
dc.subject |
0605 Microbiology |
|
dc.subject |
1107 Immunology |
|
dc.subject |
1108 Medical Microbiology |
|
dc.title |
Effector loss drives adaptation of Pseudomonas syringae pv. actinidiae biovar 3 to Actinidia arguta. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1371/journal.ppat.1010542 |
|
pubs.issue |
5 |
|
pubs.begin-page |
e1010542 |
|
pubs.volume |
18 |
|
dc.date.updated |
2022-06-15T01:45:03Z |
|
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
35622878 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/35622878 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Journal Article |
|
pubs.elements-id |
906893 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
dc.identifier.eissn |
1553-7374 |
|
dc.identifier.pii |
PPATHOGENS-D-21-02462 |
|
pubs.record-created-at-source-date |
2022-06-15 |
|
pubs.online-publication-date |
2022-05-27 |
|