dc.contributor.author |
Ryu, Kwon-Yul |
|
dc.contributor.author |
Maehr, René |
|
dc.contributor.author |
Gilchrist, Catherine A |
|
dc.contributor.author |
Long, Michael A |
|
dc.contributor.author |
Bouley, Donna M |
|
dc.contributor.author |
Mueller, Britta |
|
dc.contributor.author |
Ploegh, Hidde L |
|
dc.contributor.author |
Kopito, Ron R |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2022-07-27T22:34:06Z |
|
dc.date.available |
2022-07-27T22:34:06Z |
|
dc.date.issued |
2007-06 |
|
dc.identifier.citation |
(2007). The EMBO Journal, 26(11), 2693-2706. |
|
dc.identifier.issn |
0261-4189 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/60619 |
|
dc.description.abstract |
UbC is one of two stress-inducible polyubiquitin genes in mammals and is thought to supplement the constitutive UbA genes in maintaining cellular ubiquitin (Ub) levels during episodes of cellular stress. We have generated mice harboring a targeted disruption of the UbC gene. UbC(-/-) embryos die between embryonic days 12.5 and 14.5 in utero, most likely owing to a severe defect in liver cell proliferation. Mouse embryonic fibroblasts from UbC(-/-) embryos exhibit reduced growth rates, premature senescence, increased apoptosis and delayed cell-cycle progression, with slightly, but significantly, decreased steady-state Ub levels. UbC(-/-) fibroblasts are hypersensitive to proteasome inhibitors and heat shock, and unable to adequately increase Ub levels in response to these cellular stresses. Most, but not all of the UbC(-/-) phenotypes can be rescued by providing additional Ub from a poly hemagglutinin-tagged Ub minigene expressed from the Hprt locus. We propose that UbC is regulated by a process that senses Ub pool dynamics. These data establish that UbC constitutes an essential source of Ub during cell proliferation and stress that cannot be compensated by other Ub genes. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Wiley |
|
dc.relation.ispartofseries |
The EMBO journal |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Liver |
|
dc.subject |
Fibroblasts |
|
dc.subject |
Animals |
|
dc.subject |
Mice, Knockout |
|
dc.subject |
Mice |
|
dc.subject |
Ubiquitin C |
|
dc.subject |
Cell Cycle |
|
dc.subject |
Cell Proliferation |
|
dc.subject |
Gene Expression Regulation |
|
dc.subject |
Fetal Development |
|
dc.subject |
Genes, Essential |
|
dc.subject |
Cellular Senescence |
|
dc.subject |
Digestive Diseases |
|
dc.subject |
Liver Disease |
|
dc.subject |
Genetics |
|
dc.subject |
Rare Diseases |
|
dc.subject |
1 Underpinning research |
|
dc.subject |
1.1 Normal biological development and functioning |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Cell Biology |
|
dc.subject |
cellular stress |
|
dc.subject |
gene expression |
|
dc.subject |
liver development |
|
dc.subject |
polyubiquitin |
|
dc.subject |
ubiquitin |
|
dc.subject |
MULTI-UBIQUITIN CHAINS |
|
dc.subject |
PROTEIN-DEGRADATION |
|
dc.subject |
DEUBIQUITINATING ENZYMES |
|
dc.subject |
MESSENGER-RNA |
|
dc.subject |
EXPRESSION |
|
dc.subject |
MONOUBIQUITIN |
|
dc.subject |
DISRUPTION |
|
dc.subject |
APOPTOSIS |
|
dc.subject |
PROMOTER |
|
dc.subject |
REVEALS |
|
dc.subject |
0604 Genetics |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.subject |
Biomedical |
|
dc.subject |
Basic Science |
|
dc.subject |
06 Biological Sciences |
|
dc.subject |
08 Information and Computing Sciences |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
The mouse polyubiquitin gene UbC is essential for fetal liver development, cell-cycle progression and stress tolerance. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1038/sj.emboj.7601722 |
|
pubs.issue |
11 |
|
pubs.begin-page |
2693 |
|
pubs.volume |
26 |
|
dc.date.updated |
2022-06-16T04:23:40Z |
|
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.pmid |
17491588 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/17491588 |
|
pubs.end-page |
2706 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Comparative Study |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.subtype |
Research Support, N.I.H., Extramural |
|
pubs.elements-id |
217330 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
School of Medicine |
|
pubs.org-id |
Paediatrics Child & Youth Hlth |
|
dc.identifier.eissn |
1460-2075 |
|
dc.identifier.pii |
7601722 |
|
pubs.record-created-at-source-date |
2022-06-16 |
|
pubs.online-publication-date |
2007-06-06 |
|