dc.contributor.author |
Pochon, Xavier |
|
dc.contributor.author |
Zaiko, Anastasija |
|
dc.contributor.author |
Hopkins, Grant A |
|
dc.contributor.author |
Banks, Jonathan C |
|
dc.contributor.author |
Wood, Susanna A |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2022-09-19T23:17:27Z |
|
dc.date.available |
2022-09-19T23:17:27Z |
|
dc.date.issued |
2015-01 |
|
dc.identifier.citation |
(2015). Biofouling: the journal of bioadhesion and biofilm research, 31(3), 241-251. |
|
dc.identifier.issn |
0892-7014 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/61338 |
|
dc.description.abstract |
Marine biofilms are precursors for colonization by larger fouling organisms, including non-indigenous species (NIS). In this study, high-throughput sequencing (HTS) of 18S rRNA metabarcodes was used to investigate four sampling methods (modified syringe, sterilized sponge, underwater tape and sterilized swab) for characterizing eukaryotic communities in marine biofilms. Perspex™ plates were sampled in and out of water. DNA collected with tape did not amplify. Otherwise, there were no statistical differences in communities among the remaining three sampling devices or between the two environments. Sterilized sponges are recommended for ease of use underwater. In-depth HTS analysis identified diverse eukaryotic communities, dominated by Metazoa and Chromoalveolata. Among the latter, diatoms (Bacillariophyceae) were particularly abundant (33% of reads assigned to Chromalveolata). The NIS Ciona savignyi was detected in all samples. The application of HTS in marine biofilm surveillance could facilitate early detection of NIS, improving the probability of successful eradication. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Taylor & Francis |
|
dc.relation.ispartofseries |
Biofouling |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Animals |
|
dc.subject |
Urochordata |
|
dc.subject |
Diatoms |
|
dc.subject |
Biofilms |
|
dc.subject |
DNA |
|
dc.subject |
RNA, Ribosomal, 18S |
|
dc.subject |
Sequence Analysis, DNA |
|
dc.subject |
Computational Biology |
|
dc.subject |
Biodiversity |
|
dc.subject |
Seawater |
|
dc.subject |
Phylogeny |
|
dc.subject |
DNA Barcoding, Taxonomic |
|
dc.subject |
High-Throughput Nucleotide Sequencing |
|
dc.subject |
18S rRNA gene |
|
dc.subject |
Ciona savignyi |
|
dc.subject |
High-throughput sequencing (HTS) |
|
dc.subject |
non-indigenous species (NIS) |
|
dc.subject |
operational taxonomic unit (OTU) |
|
dc.subject |
underwater sampling devices |
|
dc.subject |
Biotechnology |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biotechnology & Applied Microbiology |
|
dc.subject |
Marine & Freshwater Biology |
|
dc.subject |
CIONA-SAVIGNYI |
|
dc.subject |
SURFACES |
|
dc.subject |
DIATOM |
|
dc.subject |
RECOMMENDATIONS |
|
dc.subject |
HETEROGENEITY |
|
dc.subject |
SENSITIVITY |
|
dc.subject |
SETTLEMENT |
|
dc.subject |
INHIBITION |
|
dc.subject |
MANAGEMENT |
|
dc.subject |
0605 Microbiology |
|
dc.subject |
05 Environmental Sciences |
|
dc.subject |
06 Biological Sciences |
|
dc.subject |
10 Technology |
|
dc.title |
Early detection of eukaryotic communities from marine biofilm using high-throughput sequencing: an assessment of different sampling devices. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1080/08927014.2015.1028923 |
|
pubs.issue |
3 |
|
pubs.begin-page |
241 |
|
pubs.volume |
31 |
|
dc.date.updated |
2022-08-13T07:12:46Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
25877857 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/25877857 |
|
pubs.end-page |
251 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
486248 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Marine Science |
|
dc.identifier.eissn |
1029-2454 |
|
pubs.record-created-at-source-date |
2022-08-13 |
|
pubs.online-publication-date |
2015-04-16 |
|