dc.contributor.advisor |
McGlashan, Sue |
|
dc.contributor.advisor |
Hisey, Colin |
|
dc.contributor.advisor |
Chhana, Ashika |
|
dc.contributor.author |
Narula, Varima Renu |
|
dc.date.accessioned |
2022-11-23T20:21:24Z |
|
dc.date.available |
2022-11-23T20:21:24Z |
|
dc.date.issued |
2022 |
en |
dc.identifier.uri |
https://hdl.handle.net/2292/61923 |
|
dc.description.abstract |
There is increasing evidence that osteoarthritis (OA) is caused by an active process of
inflammation and degeneration, rather than a passive age-related process of wear and tear
as previously thought. Obesity (BMI ≥ 30) not only increases the overall risk of developing
knee OA by approximately two-fold, but also increases the severity of disease. New Zealand
has the third highest rates for obesity in the Organisation for Economic Cooperation and
Development (OECD) countries, and as a result there is an increased incidence of OA in NZ
associated with the increased BMI. Obesity-associated OA has been recognized as a subtype
of OA having specific disease pathology and risk factors, and obesity impacts OA beyond its
weight-bearing implications, as obese individuals show a higher prevalence of OA even in nonweight-bearing joints like the wrist and hand. It can also lead to chronic systemic
inflammation but how this translates to the joints, and the molecular mechanisms underlying
the association between the obesity related soluble factors and the metabolic complications
that have been implicated in OA pathogenesis have not been studied or understood fully to
date. For instance, the means by which different tissues within the joint communicate with
each other, such as extracellular vesicles, may have significant effects on the disease
progression, encouraging the study of the OA joint in its totality.
Extracellular Vesicles (EVs) carry proinflammatory molecules in their cargo which can alter
during development of OA and might be influenced by obesity-associated systemic
inflammation. EVs are found in synovial fluid (SF), articular cartilage and in the supernatants
of synovial cells and chondrocytes. As inflammatory changes in joint tissues may be mediated
by EVs present in the SF, SF EVs in arthritic joints could contribute to anomalous gene
expression and alterations in the extracellular matrix of the articular cartilage. Thus, the
characteristics and the molecular contents within EVs might be involved in the increased
inflammatory signalling seen within the joints of the OA patients with obesity. The research
outlined in this study aims to understand the differences in the OA subtypes by characterising
biochemical and proteomic contents of SF EVs from patients with knee OA, with or without
obesity to understand their role in the development of knee OA in people with obesity. |
|
dc.publisher |
ResearchSpace@Auckland |
en |
dc.relation.ispartof |
Masters Thesis - University of Auckland |
en |
dc.relation.isreferencedby |
UoA |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-nd/3.0/nz/ |
|
dc.title |
Investigating the properties of synovial fluid extracellular vesicles from patients with knee osteoarthritis and with or without obesity |
|
dc.type |
Thesis |
en |
thesis.degree.discipline |
Biomedical Science |
|
thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Masters |
en |
dc.date.updated |
2022-10-28T00:56:00Z |
|
dc.rights.holder |
Copyright: the author |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |