Replacement of the Acrid tert‐Butylthiol and an Improved Isolation Protocol for Cysteine Lipidation on a Peptide or Amino Acid (CLipPA)

Yang, Sung‐Hyun; Hermant, Yann OJ; Harris, Paul WR; Brimble, Margaret A

dc.contributor.author	Yang, Sung‐Hyun
dc.contributor.author	Hermant, Yann OJ
dc.contributor.author	Harris, Paul WR
dc.contributor.author	Brimble, Margaret A
dc.date.accessioned	2023-03-06T03:46:22Z
dc.date.available	2023-03-06T03:46:22Z
dc.date.issued	2020-02-28
dc.identifier.citation	(2020). European Journal of Organic Chemistry, 2020(8), 944-947.
dc.identifier.issn	1434-193X
dc.identifier.uri	https://hdl.handle.net/2292/63134
dc.description.abstract	Lipidation is a key modification affecting the physiochemical properties of peptides and widely used in the design of drug candidates. Cysteine Lipidation on a Peptide or Amino Acid (CLipPA) enables rapid synthesis of a library of S-lipidated peptides via a thiol-ene reaction between a free sulfhydryl group on cysteine-containing peptide and a vinyl ester bearing a lipid. Optimization of the CLipPA procedure was performed by replacing the malodorous tert-butylthiol and modifying the work-up procedure to facilitate the analysis and isolation of lipopeptides.
dc.language	en
dc.publisher	Wiley
dc.relation.ispartofseries	European Journal of Organic Chemistry
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
dc.subject	Science & Technology
dc.subject	Physical Sciences
dc.subject	Chemistry, Organic
dc.subject	Chemistry
dc.subject	Lipidation
dc.subject	Peptides
dc.subject	Thiol-ene
dc.subject	Cysteine
dc.subject	ANTIBIOTICS
dc.subject	CGRP
dc.subject	0304 Medicinal and Biomolecular Chemistry
dc.subject	0305 Organic Chemistry
dc.title	Replacement of the Acrid tert‐Butylthiol and an Improved Isolation Protocol for Cysteine Lipidation on a Peptide or Amino Acid (CLipPA)
dc.type	Journal Article
dc.identifier.doi	10.1002/ejoc.201901696
pubs.issue	8
pubs.begin-page	944
pubs.volume	2020
dc.date.updated	2023-02-28T07:14:47Z
dc.rights.holder	Copyright: The authors	en
pubs.author-url	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000513728700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d
pubs.end-page	947
pubs.publication-status	Published
dc.rights.accessrights	http://purl.org/eprint/accessRights/RetrictedAccess	en
pubs.subtype	Article
pubs.subtype	Journal
pubs.elements-id	795992
pubs.org-id	Science
pubs.org-id	Chemistry
pubs.org-id	Science Research
pubs.org-id	Maurice Wilkins Centre (2010-2014)
dc.identifier.eissn	1099-0690
pubs.record-created-at-source-date	2023-02-28
pubs.online-publication-date	2020-02-17