dc.contributor.author |
Mugisho, Odunayo O |
|
dc.contributor.author |
Rupenthal, Ilva D |
|
dc.contributor.author |
Squirrell, David M |
|
dc.contributor.author |
Bould, Sarah J |
|
dc.contributor.author |
Danesh-Meyer, Helen V |
|
dc.contributor.author |
Zhang, Jie |
|
dc.contributor.author |
Green, Colin R |
|
dc.contributor.author |
Acosta, Monica L |
|
dc.contributor.editor |
Lewin, Alfred S |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2023-03-09T22:23:15Z |
|
dc.date.available |
2023-03-09T22:23:15Z |
|
dc.date.issued |
2018-01 |
|
dc.identifier.citation |
(2018). PLoS One, 13(8), e0202156-. |
|
dc.identifier.issn |
1932-6203 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/63266 |
|
dc.description.abstract |
Diabetic retinopathy is a vascular disease of the retina characterised by hyperglycaemic and inflammatory processes. Most animal models of diabetic retinopathy are hyperglycaemia-only models that do not account for the significant role that inflammation plays in the development of the disease. In the present study, we present data on the establishment of a new animal model of diabetic retinopathy that incorporates both hyperglycaemia and inflammation. We hypothesized that inflammation may trigger and worsen the development of diabetic retinopathy in a hyperglycaemic environment. Pro-inflammatory cytokines, IL-1β and TNF-α, were therefore injected into the vitreous of non-obese diabetic (NOD) mice. CD1 mice were used as same genetic background controls. Fundus and optical coherence tomography images were obtained before (day 0) as well as on days 2 and 7 after intravitreal cytokine injection to assess vessel dilation and beading, retinal and vitreous hyper-reflective foci and retinal thickness. Astrogliosis and microgliosis were assessed using immunohistochemistry. Results showed that intravitreal cytokines induced vessel dilation, beading, severe vitreous hyper-reflective foci, retinal oedema, increased astrogliosis and microglia upregulation in diabetic NOD mice. Intravitreal injection of inflammatory cytokines into the eyes of diabetic mice therefore appears to provide a new model of diabetic retinopathy that could be used for the study of disease progression and treatment strategies. |
|
dc.format.medium |
Electronic-eCollection |
|
dc.language |
eng |
|
dc.publisher |
Public Library of Science (PLoS) |
|
dc.relation.ispartofseries |
PloS one |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Animals |
|
dc.subject |
Mice, Inbred NOD |
|
dc.subject |
Mice |
|
dc.subject |
Diabetic Retinopathy |
|
dc.subject |
Diabetes Mellitus, Experimental |
|
dc.subject |
Disease Models, Animal |
|
dc.subject |
Blood Glucose |
|
dc.subject |
Inflammation Mediators |
|
dc.subject |
Cytokines |
|
dc.subject |
Tomography, Optical Coherence |
|
dc.subject |
Immunohistochemistry |
|
dc.subject |
Severity of Illness Index |
|
dc.subject |
Models, Biological |
|
dc.subject |
Female |
|
dc.subject |
Intravitreal Injections |
|
dc.subject |
Biomarkers |
|
dc.subject |
Eye Disease and Disorders of Vision |
|
dc.subject |
Diabetes |
|
dc.subject |
2.1 Biological and endogenous factors |
|
dc.subject |
2 Aetiology |
|
dc.subject |
Eye |
|
dc.subject |
Metabolic and endocrine |
|
dc.subject |
Science & Technology |
|
dc.subject |
Multidisciplinary Sciences |
|
dc.subject |
Science & Technology - Other Topics |
|
dc.subject |
BLOOD-RETINAL BARRIER |
|
dc.subject |
MACULAR-EDEMA |
|
dc.subject |
HYPERREFLECTIVE FOCI |
|
dc.subject |
MOUSE MODEL |
|
dc.subject |
NOD MICE |
|
dc.subject |
CELLS |
|
dc.subject |
DEATH |
|
dc.subject |
PATHOPHYSIOLOGY |
|
dc.subject |
HYPERGLYCEMIA |
|
dc.subject |
PERSPECTIVES |
|
dc.subject |
1103 Clinical Sciences |
|
dc.subject |
1113 Opthalmology and Optometry |
|
dc.subject |
Biomedical |
|
dc.subject |
Clinical Medicine and Science |
|
dc.title |
Intravitreal pro-inflammatory cytokines in non-obese diabetic mice: Modelling signs of diabetic retinopathy. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1371/journal.pone.0202156 |
|
pubs.issue |
8 |
|
pubs.begin-page |
e0202156 |
|
pubs.volume |
13 |
|
dc.date.updated |
2023-02-18T09:05:57Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
30133488 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/30133488 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
752375 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Science |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
School of Medicine |
|
pubs.org-id |
Ophthalmology Department |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
pubs.org-id |
Optometry and Vision Science |
|
dc.identifier.eissn |
1932-6203 |
|
dc.identifier.pii |
PONE-D-18-16252 |
|
pubs.number |
ARTN e0202156 |
|
pubs.record-created-at-source-date |
2023-02-18 |
|
pubs.online-publication-date |
2018-08-22 |
|