dc.contributor.author |
Pearson, CK |
|
dc.contributor.author |
Wilson, SB |
|
dc.contributor.author |
Schaffer, R |
|
dc.contributor.author |
Ross, AW |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2023-08-02T03:54:50Z |
|
dc.date.available |
2023-08-02T03:54:50Z |
|
dc.date.issued |
1993-12 |
|
dc.identifier.citation |
(1993). The Federation of European Biochemical Societies (FEBS) Journal, 218(2), 397-404. |
|
dc.identifier.issn |
0014-2956 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/65220 |
|
dc.description.abstract |
NAD is normally regarded as a redox molecule or as the substrate for ADP-ribosylation reactions. In this study, we describe the rapid metabolism of NAD by Percoll-gradient-purified lettuce chloroplasts and show that the adenine moiety can be incorporated into RNA in a dark-activated reaction that senses the redox state of the cytochrome b6f complex. Isolated chloroplasts rapidly metabolised radiolabelled NAD+ to 5'-AMP (within seconds) and adenosine during a 60-min incubation in vitro; the products were analysed by high-performance liquid chromatography. No radiolabelled ADP-ribose was detected. Radioactivity was incorporated into trichloroacetic-acid-insoluble material during this period, with approximately 2-4-fold more incorporation occurring in the dark. Most of this radiolabel was rendered acid-soluble by dilute alkaline digestion at 37 degrees C, yielding an approximately equal mixture of 2'-AMP and 3'-AMP, and by RNase digestion, identifying the acid-insoluble radioactive material as RNA. Protein-bound ADP-ribose would have yielded 5'-AMP and/or oligomeric/polymeric ADP-ribose after alkali digestion. The utilisation of NAD metabolites for RNA synthesis was restricted to the thylakoid compartment of the chloroplast. The use of a variety of electron-transport inhibitors such as 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, bromanil (tetrabromo-1,4-benzoquinone), electron donors (dithiothreitol), electron acceptors (ferricyanide) and an uncoupler showed that the incorporation of radiolabel from NAD into acid-insoluble material was favoured when the cytochrome b6f complex was in the oxidised state (as pertaining to incubations in the dark). |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Wiley |
|
dc.relation.ispartofseries |
European journal of biochemistry |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Chloroplasts |
|
dc.subject |
Radioisotopes |
|
dc.subject |
NAD |
|
dc.subject |
Cytochromes |
|
dc.subject |
Cytochromes f |
|
dc.subject |
RNA |
|
dc.subject |
Chromatography, High Pressure Liquid |
|
dc.subject |
Electron Transport |
|
dc.subject |
Oxidation-Reduction |
|
dc.subject |
Kinetics |
|
dc.subject |
Hydrogen-Ion Concentration |
|
dc.subject |
Light |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
RAT-LIVER MITOCHONDRIA |
|
dc.subject |
ADP-RIBOSYLATION |
|
dc.subject |
PROTEIN-PHOSPHORYLATION |
|
dc.subject |
RIBONUCLEIC-ACID |
|
dc.subject |
POLY(ADP-RIBOSE) |
|
dc.subject |
POLYPEPTIDES |
|
dc.subject |
MEMBRANE |
|
dc.subject |
0303 Macromolecular and Materials Chemistry |
|
dc.subject |
0304 Medicinal and Biomolecular Chemistry |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.subject |
1101 Medical Biochemistry and Metabolomics |
|
dc.subject |
3101 Biochemistry and cell biology |
|
dc.subject |
3205 Medical biochemistry and metabolomics |
|
dc.subject |
3404 Medicinal and biomolecular chemistry |
|
dc.title |
NAD turnover and utilisation of metabolites for RNA synthesis in a reaction sensing the redox state of the cytochrome b6f complex in isolated chloroplasts. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1111/j.1432-1033.1993.tb18389.x |
|
pubs.issue |
2 |
|
pubs.begin-page |
397 |
|
pubs.volume |
218 |
|
dc.date.updated |
2023-07-23T22:45:39Z |
|
dc.rights.holder |
Copyright: FEBS |
en |
dc.identifier.pmid |
7505745 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/7505745 |
|
pubs.end-page |
404 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RetrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
223092 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
dc.identifier.eissn |
1432-1033 |
|
pubs.record-created-at-source-date |
2023-07-24 |
|
pubs.online-publication-date |
2005-03-03 |
|