Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence.

Show simple item record Aghababa, Haniyeh Loh, Jacelyn MS Proft, Thomas
dc.coverage.spatial United States 2023-08-02T23:48:08Z 2023-08-02T23:48:08Z 2023-01
dc.identifier.citation (2023). Methods in Molecular Biology, 2674, 119-129.
dc.identifier.issn 1064-3745
dc.description.abstract Group A Streptococcus (GAS, Streptococcus pyogenes) is an exclusively human pathogen that causes a range of diseases, including pharyngitis, tonsillitis, impetigo, erysipelas, necrotizing fasciitis, and toxic shock syndrome. Post-streptococcal sequelae include acute rheumatic fever and rheumatic heart disease. The bacterium produces a large arsenal of virulence factors that contribute to host tissue adhesion/colonization, bacterial spread, and host immune evasion. Immune evasion factors include proteins that interfere with complement, a system of plasma proteins that are activated by pathogens resulting in a variety of reactions on the surface of the pathogen. This leads to the activation of active components with a variety of effector functions, such as cell lysis, opsonization, and chemotaxis of phagocytes to the site of infection. We have recently identified a novel "complement evasion factor" (CEF) in S. pyogenes. CEF directly interacts with complement proteins C1r, C1s, C3, and C5, interrupts all three complement pathways, and prevents opsonization of the bacterial surface with C3b. We here present methods used to analyze the complement interference of CEF.
dc.format.medium Print
dc.language eng
dc.publisher Springer Nature
dc.relation.ispartofseries Methods in molecular biology (Clifton, N.J.)
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
dc.subject Humans
dc.subject Streptococcus pyogenes
dc.subject Streptococcal Infections
dc.subject Bacterial Proteins
dc.subject Virulence Factors
dc.subject Immunologic Factors
dc.subject Virulence
dc.subject Complement System Proteins
dc.subject Complement evasion
dc.subject Complement proteins
dc.subject Galleria mellonella infection model
dc.subject Group A Streptococcus
dc.subject Virulence mechanisms
dc.subject Rare Diseases
dc.subject Infectious Diseases
dc.subject Arthritis
dc.subject Emerging Infectious Diseases
dc.subject Foodborne Illness
dc.subject Prevention
dc.subject 2.2 Factors relating to the physical environment
dc.subject 2.1 Biological and endogenous factors
dc.subject 2 Aetiology
dc.subject Infection
dc.subject 0399 Other Chemical Sciences
dc.subject 0601 Biochemistry and Cell Biology
dc.title Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence.
dc.type Journal Article
dc.identifier.doi 10.1007/978-1-0716-3243-7_8
pubs.begin-page 119
pubs.volume 2674 2023-07-06T05:07:48Z
dc.rights.holder Copyright: The authors en
dc.identifier.pmid 37258964 (pubmed)
pubs.end-page 129
pubs.publication-status Published
dc.rights.accessrights en
pubs.subtype Journal Article
pubs.elements-id 964193 Medical and Health Sciences Science Science Research Medical Sciences Molecular Medicine Maurice Wilkins Centre (2010-2014)
dc.identifier.eissn 1940-6029
pubs.record-created-at-source-date 2023-07-06 2023-06-01

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