dc.contributor.author |
Aghababa, Haniyeh |
|
dc.contributor.author |
Loh, Jacelyn MS |
|
dc.contributor.author |
Proft, Thomas |
|
dc.coverage.spatial |
United States |
|
dc.date.accessioned |
2023-08-02T23:48:08Z |
|
dc.date.available |
2023-08-02T23:48:08Z |
|
dc.date.issued |
2023-01 |
|
dc.identifier.citation |
(2023). Methods in Molecular Biology, 2674, 119-129. |
|
dc.identifier.issn |
1064-3745 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/65266 |
|
dc.description.abstract |
Group A Streptococcus (GAS, Streptococcus pyogenes) is an exclusively human pathogen that causes a range of diseases, including pharyngitis, tonsillitis, impetigo, erysipelas, necrotizing fasciitis, and toxic shock syndrome. Post-streptococcal sequelae include acute rheumatic fever and rheumatic heart disease. The bacterium produces a large arsenal of virulence factors that contribute to host tissue adhesion/colonization, bacterial spread, and host immune evasion. Immune evasion factors include proteins that interfere with complement, a system of plasma proteins that are activated by pathogens resulting in a variety of reactions on the surface of the pathogen. This leads to the activation of active components with a variety of effector functions, such as cell lysis, opsonization, and chemotaxis of phagocytes to the site of infection. We have recently identified a novel "complement evasion factor" (CEF) in S. pyogenes. CEF directly interacts with complement proteins C1r, C1s, C3, and C5, interrupts all three complement pathways, and prevents opsonization of the bacterial surface with C3b. We here present methods used to analyze the complement interference of CEF. |
|
dc.format.medium |
Print |
|
dc.language |
eng |
|
dc.publisher |
Springer Nature |
|
dc.relation.ispartofseries |
Methods in molecular biology (Clifton, N.J.) |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Humans |
|
dc.subject |
Streptococcus pyogenes |
|
dc.subject |
Streptococcal Infections |
|
dc.subject |
Bacterial Proteins |
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dc.subject |
Virulence Factors |
|
dc.subject |
Immunologic Factors |
|
dc.subject |
Virulence |
|
dc.subject |
Complement System Proteins |
|
dc.subject |
Complement evasion |
|
dc.subject |
Complement proteins |
|
dc.subject |
Galleria mellonella infection model |
|
dc.subject |
Group A Streptococcus |
|
dc.subject |
Virulence mechanisms |
|
dc.subject |
Rare Diseases |
|
dc.subject |
Infectious Diseases |
|
dc.subject |
Arthritis |
|
dc.subject |
Emerging Infectious Diseases |
|
dc.subject |
Foodborne Illness |
|
dc.subject |
Prevention |
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dc.subject |
2.2 Factors relating to the physical environment |
|
dc.subject |
2.1 Biological and endogenous factors |
|
dc.subject |
2 Aetiology |
|
dc.subject |
Infection |
|
dc.subject |
0399 Other Chemical Sciences |
|
dc.subject |
0601 Biochemistry and Cell Biology |
|
dc.title |
Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1007/978-1-0716-3243-7_8 |
|
pubs.begin-page |
119 |
|
pubs.volume |
2674 |
|
dc.date.updated |
2023-07-06T05:07:48Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
37258964 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/37258964 |
|
pubs.end-page |
129 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RetrictedAccess |
en |
pubs.subtype |
Journal Article |
|
pubs.elements-id |
964193 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Science |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
Medical Sciences |
|
pubs.org-id |
Molecular Medicine |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1940-6029 |
|
pubs.record-created-at-source-date |
2023-07-06 |
|
pubs.online-publication-date |
2023-06-01 |
|