dc.contributor.author |
Yang, Panzao |
|
dc.contributor.author |
Davidson, Joanne O |
|
dc.contributor.author |
Zhou, Kelly Q |
|
dc.contributor.author |
Wilson, Rani |
|
dc.contributor.author |
Wassink, Guido |
|
dc.contributor.author |
Prasad, Jaya D |
|
dc.contributor.author |
Bennet, Laura |
|
dc.contributor.author |
Gunn, Alistair J |
|
dc.contributor.author |
Dean, Justin M |
|
dc.coverage.spatial |
Switzerland |
|
dc.date.accessioned |
2023-10-05T02:06:21Z |
|
dc.date.available |
2023-10-05T02:06:21Z |
|
dc.date.issued |
2023-02 |
|
dc.identifier.citation |
(2023). International Journal of Molecular Sciences, 24(4), 3706-. |
|
dc.identifier.issn |
1422-0067 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/66135 |
|
dc.description.abstract |
Therapeutic hypothermia significantly improves outcomes after neonatal hypoxic-ischemic (HI) encephalopathy but is only partially protective. There is evidence that cortical inhibitory interneuron circuits are particularly vulnerable to HI and that loss of interneurons may be an important contributor to long-term neurological dysfunction in these infants. In the present study, we examined the hypothesis that the duration of hypothermia has differential effects on interneuron survival after HI. Near-term fetal sheep received sham ischemia or cerebral ischemia for 30 min, followed by cerebral hypothermia from 3 h after ischemia end and continued up to 48 h, 72 h, or 120 h recovery. Sheep were euthanized after 7 days for histology. Hypothermia up to 48 h recovery resulted in moderate neuroprotection of glutamate decarboxylase (GAD)+ and parvalbumin+ interneurons but did not improve survival of calbindin+ cells. Hypothermia up to 72 h recovery was associated with significantly increased survival of all three interneuron phenotypes compared with sham controls. By contrast, while hypothermia up to 120 h recovery did not further improve (or impair) GAD+ or parvalbumin+ neuronal survival compared with hypothermia up to 72 h, it was associated with decreased survival of calbindin+ interneurons. Finally, protection of parvalbumin+ and GAD+ interneurons, but not calbindin+ interneurons, with hypothermia was associated with improved recovery of electroencephalographic (EEG) power and frequency by day 7 after HI. The present study demonstrates differential effects of increasing the duration of hypothermia on interneuron survival after HI in near-term fetal sheep. These findings may contribute to the apparent preclinical and clinical lack of benefit of very prolonged hypothermia. |
|
dc.format.medium |
Electronic |
|
dc.language |
eng |
|
dc.publisher |
MDPI |
|
dc.relation.ispartofseries |
International journal of molecular sciences |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Interneurons |
|
dc.subject |
Animals |
|
dc.subject |
Sheep |
|
dc.subject |
Cerebral Infarction |
|
dc.subject |
Hypoxia-Ischemia, Brain |
|
dc.subject |
Ischemia |
|
dc.subject |
Parvalbumins |
|
dc.subject |
Hypothermia, Induced |
|
dc.subject |
EEG |
|
dc.subject |
cerebral cortex |
|
dc.subject |
cooling |
|
dc.subject |
interneuron |
|
dc.subject |
neonatal |
|
dc.subject |
therapeutic hypothermia |
|
dc.subject |
3101 Biochemistry and Cell Biology |
|
dc.subject |
31 Biological Sciences |
|
dc.subject |
3404 Medicinal and Biomolecular Chemistry |
|
dc.subject |
34 Chemical Sciences |
|
dc.subject |
3107 Microbiology |
|
dc.subject |
Neurosciences |
|
dc.subject |
Brain Disorders |
|
dc.subject |
Pediatric |
|
dc.subject |
Stroke |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Physical Sciences |
|
dc.subject |
Biochemistry & Molecular Biology |
|
dc.subject |
Chemistry, Multidisciplinary |
|
dc.subject |
Chemistry |
|
dc.subject |
CALCIUM-BINDING PROTEINS |
|
dc.subject |
GABAERGIC INTERNEURONS |
|
dc.subject |
PARVALBUMIN IMMUNOREACTIVITY |
|
dc.subject |
NEURONAL SUBPOPULATIONS |
|
dc.subject |
INHIBITORY INTERNEURONS |
|
dc.subject |
POSTNATAL-DEVELOPMENT |
|
dc.subject |
NEUROTROPHIC FACTOR |
|
dc.subject |
PREFRONTAL CORTEX |
|
dc.subject |
AUDITORY-CORTEX |
|
dc.subject |
VISUAL-CORTEX |
|
dc.subject |
0399 Other Chemical Sciences |
|
dc.subject |
0604 Genetics |
|
dc.subject |
0699 Other Biological Sciences |
|
dc.title |
Therapeutic Hypothermia Attenuates Cortical Interneuron Loss after Cerebral Ischemia in Near-Term Fetal Sheep |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.3390/ijms24043706 |
|
pubs.issue |
4 |
|
pubs.begin-page |
3706 |
|
pubs.volume |
24 |
|
dc.date.updated |
2023-09-10T22:42:48Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
36835117 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/36835117 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
950608 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Medical Sciences |
|
pubs.org-id |
Physiology Division |
|
dc.identifier.eissn |
1422-0067 |
|
dc.identifier.pii |
ijms24043706 |
|
pubs.number |
ARTN 3706 |
|
pubs.record-created-at-source-date |
2023-09-11 |
|
pubs.online-publication-date |
2023-02-12 |
|