dc.contributor.author |
Yang, Cheng |
|
dc.contributor.author |
Cao, Ye |
|
dc.contributor.author |
Zhang, Yi |
|
dc.contributor.author |
Li, Long |
|
dc.contributor.author |
Xu, Ming |
|
dc.contributor.author |
Long, Yaqiu |
|
dc.contributor.author |
Rong, Ruiming |
|
dc.contributor.author |
Zhu, Tongyu |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2023-10-06T01:10:32Z |
|
dc.date.available |
2023-10-06T01:10:32Z |
|
dc.date.issued |
2015-11 |
|
dc.identifier.citation |
(2015). Journal of Translational Medicine, 13(1), 355-. |
|
dc.identifier.issn |
1479-5876 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/66176 |
|
dc.description.abstract |
Renal fibrosis is a main cause of end-stage renal disease. Clinically, there is no beneficial treatment that can effectively reverse the progressive loss of renal function. We recently synthesized a novel proteolysis-resistant cyclic helix B peptide (CHBP) that exhibits promising renoprotective effects. In this study, we evaluated the effect of CHBP on renal fibrosis in an in vivo ischemia reperfusion injury (IRI) model and in vitro TGF-β-stimulated tubular epithelial cells (TCMK-1 and HK-2) model. In the IRI in vivo model, mice were randomly divided into sham (sham operation), IR and IR + CHBP groups (n = 6). CHBP (8 nmol/kg) was administered intraperitoneally at the onset of reperfusion, and renal fibrosis was evaluated at 12 weeks post-reperfusion. Our results showed that CHBP markedly attenuated the IRI-induced deposition of collagen I and vimentin. In the in vitro model, CHBP reversed the TGF-β-induced down-regulation of E-cadherin and up-regulation of α-SMA and vimentin. Furthermore, CHBP inhibited the phosphorylation of Akt and Forkhead box O 3a (FoxO3a), whose anti-fibrotic effect could be reversed by the 3-phosphoinositide-dependent kinase-1 (PI3K) inhibitor wortmannin as well as FoxO3a siRNA. These findings demonstrate that CHBP attenuates renal fibrosis and the epithelial-mesenchymal transition of tubular cells, possibly through suppression of the PI3K/Akt pathway and thereby the inhibition FoxO3a activity. |
|
dc.format.medium |
Electronic |
|
dc.language |
eng |
|
dc.publisher |
Springer Nature |
|
dc.relation.ispartofseries |
Journal of translational medicine |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
Kidney |
|
dc.subject |
Animals |
|
dc.subject |
Mice, Inbred BALB C |
|
dc.subject |
Humans |
|
dc.subject |
Mice |
|
dc.subject |
Kidney Diseases |
|
dc.subject |
Reperfusion Injury |
|
dc.subject |
Fibrosis |
|
dc.subject |
Peptides, Cyclic |
|
dc.subject |
Transforming Growth Factor beta |
|
dc.subject |
Male |
|
dc.subject |
Proto-Oncogene Proteins c-akt |
|
dc.subject |
Forkhead Transcription Factors |
|
dc.subject |
Phosphatidylinositol 3-Kinases |
|
dc.subject |
Epithelial-Mesenchymal Transition |
|
dc.subject |
Forkhead Box Protein O3 |
|
dc.subject |
32 Biomedical and Clinical Sciences |
|
dc.subject |
Kidney Disease |
|
dc.subject |
1.1 Normal biological development and functioning |
|
dc.subject |
1 Underpinning research |
|
dc.subject |
2 Aetiology |
|
dc.subject |
2.1 Biological and endogenous factors |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Medicine, Research & Experimental |
|
dc.subject |
Research & Experimental Medicine |
|
dc.subject |
Cyclic helix B peptide |
|
dc.subject |
Renal ischemia reperfusion injury |
|
dc.subject |
Akt |
|
dc.subject |
FoxO3 |
|
dc.subject |
NAKED CASPASE-3 SIRNA |
|
dc.subject |
ACUTE KIDNEY INJURY |
|
dc.subject |
ISCHEMIA/REPERFUSION INJURY |
|
dc.subject |
AUTOTRANSPLANT KIDNEYS |
|
dc.subject |
INFLAMMATION |
|
dc.subject |
ACTIVATION |
|
dc.subject |
DISEASE |
|
dc.subject |
MODEL |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.subject |
42 Health sciences |
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dc.title |
Cyclic helix B peptide inhibits ischemia reperfusion-induced renal fibrosis via the PI3K/Akt/FoxO3a pathway |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1186/s12967-015-0699-2 |
|
pubs.issue |
1 |
|
pubs.begin-page |
355 |
|
pubs.volume |
13 |
|
dc.date.updated |
2023-09-20T22:57:15Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
26554357 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/26554357 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
953215 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Medical Sciences |
|
dc.identifier.eissn |
1479-5876 |
|
dc.identifier.pii |
10.1186/s12967-015-0699-2 |
|
pubs.number |
355 |
|
pubs.record-created-at-source-date |
2023-09-21 |
|
pubs.online-publication-date |
2015-11-10 |
|