dc.contributor.author |
Valmonte-Cortes, Gardette R |
|
dc.contributor.author |
Lilly, Sonia T |
|
dc.contributor.author |
Pearson, Michael N |
|
dc.contributor.author |
Higgins, Colleen M |
|
dc.contributor.author |
MacDiarmid, Robin M |
|
dc.coverage.spatial |
Switzerland |
|
dc.date.accessioned |
2023-12-05T20:59:06Z |
|
dc.date.available |
2023-12-05T20:59:06Z |
|
dc.date.issued |
2022-01 |
|
dc.identifier.citation |
(2022). Plants-Basel, 11(2), 188-. |
|
dc.identifier.issn |
2223-7747 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/66740 |
|
dc.description.abstract |
To our knowledge, there are no reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant virus infection. Two approaches involving molecular indicators of virus infection in the model plant Arabidopsis thaliana were examined: the accumulation of small RNAs (sRNAs) using a microfluidics-based method (Bioanalyzer); and the transcript accumulation of virus-response related host plant genes, suppressor of gene silencing 3 (AtSGS3) and calcium-dependent protein kinase 3 (AtCPK3) by reverse transcriptase-quantitative PCR (RT-qPCR). The microfluidics approach using sRNA chips has previously demonstrated good linearity and good reproducibility, both within and between chips. Good limits of detection have been demonstrated from two-fold 10-point serial dilution regression to 0.1 ng of RNA. The ratio of small RNA (sRNA) to ribosomal RNA (rRNA), as a proportion of averaged mock-inoculation, correlated with known virus infection to a high degree of certainty. AtSGS3 transcript decreased between 14- and 28-days post inoculation (dpi) for all viruses investigated, while AtCPK3 transcript increased between 14 and 28 dpi for all viruses. A combination of these two molecular approaches may be useful for assessment of virus-infection of samples without the need for diagnosis of specific virus infection. |
|
dc.format.medium |
Electronic |
|
dc.language |
eng |
|
dc.publisher |
MDPI |
|
dc.relation.ispartofseries |
Plants (Basel, Switzerland) |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
CPK |
|
dc.subject |
SGS3 |
|
dc.subject |
detection |
|
dc.subject |
host response |
|
dc.subject |
molecular markers |
|
dc.subject |
plant virus |
|
dc.subject |
sRNA |
|
dc.subject |
30 Agricultural, Veterinary and Food Sciences |
|
dc.subject |
3108 Plant Biology |
|
dc.subject |
31 Biological Sciences |
|
dc.subject |
3008 Horticultural Production |
|
dc.subject |
Genetics |
|
dc.subject |
Infectious Diseases |
|
dc.subject |
Biotechnology |
|
dc.subject |
Infection |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Plant Sciences |
|
dc.subject |
DEPENDENT PROTEIN-KINASES |
|
dc.subject |
MOSAIC-VIRUS |
|
dc.subject |
ANTIVIRAL IMMUNITY |
|
dc.subject |
RNA |
|
dc.subject |
EXPRESSION |
|
dc.subject |
DEFENSE |
|
dc.subject |
GENES |
|
dc.subject |
DIAGNOSIS |
|
dc.title |
The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected? |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.3390/plants11020188 |
|
pubs.issue |
2 |
|
pubs.begin-page |
188 |
|
pubs.volume |
11 |
|
dc.date.updated |
2023-11-19T06:01:04Z |
|
dc.rights.holder |
Copyright: The authors |
en |
dc.identifier.pmid |
35050076 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/35050076 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/OpenAccess |
en |
pubs.subtype |
research-article |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
880082 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
dc.identifier.eissn |
2223-7747 |
|
dc.identifier.pii |
plants11020188 |
|
pubs.number |
ARTN 188 |
|
pubs.record-created-at-source-date |
2023-11-19 |
|
pubs.online-publication-date |
2022-01-11 |
|