dc.contributor.author |
Feisst, Vaughan |
|
dc.contributor.author |
Kelch, Inken |
|
dc.contributor.author |
Dunn, Elliott |
|
dc.contributor.author |
Williams, Eloise |
|
dc.contributor.author |
Meidinger, Sarah |
|
dc.contributor.author |
Chen, Chun-Jen Jennifer |
|
dc.contributor.author |
Girvan, Rebecca |
|
dc.contributor.author |
Zhou, Lisa |
|
dc.contributor.author |
Sheppard, Hilary |
|
dc.contributor.author |
Locke, Michelle |
|
dc.contributor.author |
Dunbar, P Rod |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2024-06-06T02:28:30Z |
|
dc.date.available |
2024-06-06T02:28:30Z |
|
dc.date.issued |
2023-02 |
|
dc.identifier.citation |
(2023). Cytotherapy, 25(2), 174-184. |
|
dc.identifier.issn |
1465-3249 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/68619 |
|
dc.description.abstract |
<h4>Background aims</h4>The ability to culture human keratinocytes is beneficial in the treatment of skin injury and disease, as well as for testing chemicals in vitro as a substitute for animal testing.<h4>Results</h4>We have identified a novel culture medium for the rapid growth of keratinocytes from human skin. "Kelch's medium" supports keratinocyte growth that is as rapid as in the classical Rheinwald and Green method, but without the need for cholera toxin or xenogeneic feeder cells. It enables keratinocytes to out-compete co-cultured autologous fibroblasts so that separation of the epidermis from the dermis is no longer required before keratinocyte culture. Enzymatic digests of whole human skin can therefore be used to generate parallel cultures of autologous keratinocytes, fibroblasts and melanocytes simply by using different cell culture media.<h4>Conclusions</h4>This new keratinocyte medium and the simplified manufacturing procedures it enables are likely to be beneficial in skin engineering, especially for clinical applications. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
Elsevier |
|
dc.relation.ispartofseries |
Cytotherapy |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Cells, Cultured |
|
dc.subject |
Fibroblasts |
|
dc.subject |
Keratinocytes |
|
dc.subject |
Skin |
|
dc.subject |
Animals |
|
dc.subject |
Humans |
|
dc.subject |
Coculture Techniques |
|
dc.subject |
Cell Proliferation |
|
dc.subject |
cell culture |
|
dc.subject |
in vitro skin testing |
|
dc.subject |
regenerative medicine |
|
dc.subject |
skin engineering |
|
dc.subject |
3206 Medical Biotechnology |
|
dc.subject |
32 Biomedical and Clinical Sciences |
|
dc.subject |
3204 Immunology |
|
dc.subject |
Biotechnology |
|
dc.subject |
3 Good Health and Well Being |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Cell & Tissue Engineering |
|
dc.subject |
Biotechnology & Applied Microbiology |
|
dc.subject |
Cell Biology |
|
dc.subject |
Hematology |
|
dc.subject |
Medicine, Research & Experimental |
|
dc.subject |
Research & Experimental Medicine |
|
dc.subject |
HUMAN EPIDERMAL-KERATINOCYTES |
|
dc.subject |
STEM-CELLS |
|
dc.subject |
SKIN SUBSTITUTES |
|
dc.subject |
IN-VITRO |
|
dc.subject |
PROLIFERATION |
|
dc.subject |
REGENERATION |
|
dc.subject |
VIVO |
|
dc.subject |
1103 Clinical Sciences |
|
dc.title |
Rapid culture of human keratinocytes in an autologous, feeder-free system with a novel growth medium. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1016/j.jcyt.2022.09.003 |
|
pubs.issue |
2 |
|
pubs.begin-page |
174 |
|
pubs.volume |
25 |
|
dc.date.updated |
2024-05-25T03:01:17Z |
|
dc.rights.holder |
Copyright: International Society for Cell & Gene Therapy |
en |
dc.identifier.pmid |
36229300 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/36229300 |
|
pubs.end-page |
184 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RetrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
922527 |
|
pubs.org-id |
Medical and Health Sciences |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
pubs.org-id |
Science Research |
|
pubs.org-id |
School of Medicine |
|
pubs.org-id |
Surgery Department |
|
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
|
dc.identifier.eissn |
1477-2566 |
|
dc.identifier.pii |
S1465-3249(22)00785-X |
|
pubs.record-created-at-source-date |
2024-05-25 |
|
pubs.online-publication-date |
2022-10-11 |
|