dc.contributor.author |
Harford, Alice R |
|
dc.contributor.author |
Devaux, Jules BL |
|
dc.contributor.author |
Hickey, Anthony JR |
|
dc.coverage.spatial |
England |
|
dc.date.accessioned |
2024-06-09T23:28:44Z |
|
dc.date.available |
2024-06-09T23:28:44Z |
|
dc.date.issued |
2023-08 |
|
dc.identifier.citation |
(2023). Journal of Experimental Biology, 226(16), jeb245926-. |
|
dc.identifier.issn |
0022-0949 |
|
dc.identifier.uri |
https://hdl.handle.net/2292/68722 |
|
dc.description.abstract |
Oxygen is essential for most eukaryotic lifeforms, as it supports mitochondrial oxidative phosphorylation to supply ∼90% of cellular adenosine triphosphate (ATP). Fluctuations in O2 present a major stressor, with hypoxia leading to a cascade of detrimental physiological changes that alter cell operations and ultimately induce death. Nonetheless, some species episodically tolerate near-anoxic environments, and have evolved mechanisms to sustain function even during extended hypoxic periods. While mitochondria are pivotal in central metabolism, their role in hypoxia tolerance remains ill defined. Given the vulnerability of the brain to hypoxia, mitochondrial function was tested in brain homogenates of three closely related triplefin species with varying degrees of hypoxia tolerance (Bellapiscis medius, Forsterygion lapillum and Forsterygion varium). High-resolution respirometry coupled with fluorometric measurements of mitochondrial membrane potential (mtMP) permitted assessment of differences in mitochondrial function and integrity in response to intermittent hypoxia and anoxia. Traditional steady-state measures of respiratory flux and mtMP showed no differences among species. However, in the transition into anoxia, the tolerant species B. medius and F. lapillum maintained mtMP at O2 pressures 7- and 4.4-fold lower, respectively, than that of the hypoxia-sensitive F. varium and exhibited slower rates of membrane depolarisation. The results indicate that dynamic oxic-hypoxic mitochondria transitions underlie hypoxia tolerance in these intertidal fish. |
|
dc.format.medium |
Print-Electronic |
|
dc.language |
eng |
|
dc.publisher |
The Company of Biologists |
|
dc.relation.ispartofseries |
The Journal of experimental biology |
|
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
|
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
|
dc.subject |
Animals |
|
dc.subject |
Fishes |
|
dc.subject |
Oxygen |
|
dc.subject |
Adenosine Triphosphate |
|
dc.subject |
Oxidative Phosphorylation |
|
dc.subject |
Membrane Potential, Mitochondrial |
|
dc.subject |
Hypoxia |
|
dc.subject |
Anoxia |
|
dc.subject |
Membrane potential |
|
dc.subject |
Mitochondria |
|
dc.subject |
Reoxygenation |
|
dc.subject |
Respirometry |
|
dc.subject |
Triplefin fish |
|
dc.subject |
mtMP |
|
dc.subject |
3101 Biochemistry and Cell Biology |
|
dc.subject |
31 Biological Sciences |
|
dc.subject |
1.1 Normal biological development and functioning |
|
dc.subject |
1 Underpinning research |
|
dc.subject |
Science & Technology |
|
dc.subject |
Life Sciences & Biomedicine |
|
dc.subject |
Biology |
|
dc.subject |
Life Sciences & Biomedicine - Other Topics |
|
dc.subject |
HYPOXIA TOLERANCE |
|
dc.subject |
METABOLIC-RESPONSES |
|
dc.subject |
EPAULETTE SHARK |
|
dc.subject |
BRAIN |
|
dc.subject |
ATP |
|
dc.subject |
EVOLUTION |
|
dc.subject |
OVEREXPRESSION |
|
dc.subject |
TRIPTERYGIIDAE |
|
dc.subject |
MECHANISMS |
|
dc.subject |
06 Biological Sciences |
|
dc.subject |
11 Medical and Health Sciences |
|
dc.title |
Dynamic defence? Intertidal triplefin species show better maintenance of mitochondrial membrane potential than subtidal species at low oxygen pressures. |
|
dc.type |
Journal Article |
|
dc.identifier.doi |
10.1242/jeb.245926 |
|
pubs.issue |
16 |
|
pubs.begin-page |
jeb245926 |
|
pubs.volume |
226 |
|
dc.date.updated |
2024-05-08T22:31:31Z |
|
dc.rights.holder |
Copyright: The Company of Biologists Ltd |
en |
dc.identifier.pmid |
37498237 (pubmed) |
|
pubs.author-url |
https://www.ncbi.nlm.nih.gov/pubmed/37498237 |
|
pubs.publication-status |
Published |
|
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RetrictedAccess |
en |
pubs.subtype |
Research Support, Non-U.S. Gov't |
|
pubs.subtype |
Journal Article |
|
pubs.elements-id |
974008 |
|
pubs.org-id |
Science |
|
pubs.org-id |
Biological Sciences |
|
dc.identifier.eissn |
1477-9145 |
|
dc.identifier.pii |
325974 |
|
pubs.number |
ARTN jeb245926 |
|
pubs.record-created-at-source-date |
2024-05-09 |
|
pubs.online-publication-date |
2023-08-22 |
|