dc.contributor.author |
Hazell, BW |
en |
dc.contributor.author |
Te'o, VSJ |
en |
dc.contributor.author |
Bradner, JR |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.contributor.author |
Nevalainen, KMH |
en |
dc.date.accessioned |
2011-07-22T01:42:05Z |
en |
dc.date.issued |
2000-04-01 |
en |
dc.identifier.citation |
Lett Appl Microbiol 30(4):282-286 Apr 2000 |
en |
dc.identifier.issn |
0266-8254 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7003 |
en |
dc.description.abstract |
Intact conidia of three industrially relevant strains of Trichoderma reesei were effectively transformed by particle bombardment. Transformations were carried out individually with plasmids carrying either the fungal amdS or bacterial hph gene as a selectable marker and by cotransformation with both plasmids. Transformant yields with single plasmids were up to 11 stable transformants per mu g DNA at the bombardment distance of 6 cm. Mitotic stability of the transformants was 75-100% and the cotransformation efficiency averaged 92% when the first selection was performed on hygromycin B plates. The entire procedure could be completed in 1 week with the hph marker. |
en |
dc.language |
English |
en |
dc.publisher |
BLACKWELL SCIENCE LTD |
en |
dc.relation.ispartofseries |
LETTERS IN APPLIED MICROBIOLOGY |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0266-8254/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Biotechnology & Applied Microbiology |
en |
dc.subject |
Microbiology |
en |
dc.subject |
ASPERGILLUS-NIDULANS |
en |
dc.subject |
CONIDIA |
en |
dc.title |
Rapid transformation of high cellulase-producing mutant strains of Trichoderma reesei by microprojectile bombardment |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1046/j.1472-765x.2000.00715.x |
en |
pubs.issue |
4 |
en |
pubs.begin-page |
282 |
en |
pubs.volume |
30 |
en |
dc.rights.holder |
Copyright: 2000 The Society for Applied Microbiology |
en |
dc.identifier.pmid |
10792647 |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000086293000004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.end-page |
286 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
207080 |
en |
pubs.record-created-at-source-date |
2013-06-05 |
en |
pubs.dimensions-id |
10792647 |
en |