Isolation and Expression of Genes for Hemicellulases from Extremely Thermophilic Culturable and Unculturable Bacteria

Show simple item record Bergquist, Peter en Gibbs, MD en Saul, DJ en Reeves, RA en Morris, DD en Te'o, VSJ en 2011-07-24T21:38:14Z en 1998 en
dc.identifier.citation ACS Symposium Series 687:155-167 1998 en
dc.identifier.issn 0097-6156 en
dc.identifier.uri en
dc.description.abstract Our current research has focused on the isolation of genes coding for thermostable hemicellulolytic enzymes that function under alkaline conditions, and we have isolated xylanase and mannanase genes from both culturable bacteria isolated after enrichment and non-culturable organisms from consortia obtained after enhanced growth. Genomic or biomass DNA is subjected to the polymerase chain reaction (PCR) using consensus primers for xylanases or mannanases that we have designed. The full length sequences are obtained either by genomic walking PCR or by using consensus amino- and carboxy-terminal PCR primers that allow the amplification of the entire gene in a form that allows direct cloning into an expression vector. A number of novel genes have been isolated and cloned. They have been expressed in mesophilic bacteria and fungi (such as yeast and Trichoderma), since the mesophilic proteins can be removed by a simple heating step, giving a facile 20-fold purification. Selected candidate enzymes have been tested for their ability to enhance the bleaching of conventional and oxygen-bleached Pinus radiata and Eucalyptus kraft pulp. en
dc.relation.ispartofseries ACS Symposium Series en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Isolation and Expression of Genes for Hemicellulases from Extremely Thermophilic Culturable and Unculturable Bacteria en
dc.type Journal Article en
dc.identifier.doi 10.1021/bk-1998-0687.ch013 en
pubs.begin-page 155 en
pubs.volume 687 en
dc.rights.holder Copyright: 1998 American Chemical Society en
pubs.end-page 167 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 185874 en
pubs.record-created-at-source-date 2011-07-25 en

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