dc.contributor.author |
Te'O, VSJ |
en |
dc.contributor.author |
Saul, DJ |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.date.accessioned |
2011-07-24T21:40:54Z |
en |
dc.date.issued |
1995 |
en |
dc.identifier.citation |
Appl Microbiol Biotechnol 43(2):291-296 May 1995 |
en |
dc.identifier.issn |
0175-7598 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7035 |
en |
dc.description.abstract |
Caldocellum saccharolyticum is an extremely thermophilic anaerobic bacterium capable of growth on cellulose and hemicellulose as sole carbon sources. Cellulase and hemicellulase genes have been found clustered together on its genome. The gene for one of the cellulases (celA) was isolated on a λ genomic library clone, sequenced and found to comprise a large open-reading frame of 5253 base pairs that could be translated into a peptide of 1751 amino acids. To date, it is the largest cellulase gene sequenced. The translated product is a multidomain structure composed of two catalytic domains and two cellulose-binding domains linked by proline-threonine-rich regions (PT linkers). The N-terminal domain of celA encodes for an endoglucanase activity on carboxymethylcellulose, consistent with its high homology to the sequences of several other endo-1,4-β-D-glucanases. The carboxylterminal domain shows sequence homology with a cellulase from Clostridium thermocellum (CelS), which is known to act synergistically with a second component to hydrolyze crystalline cellulose. In the absence of a Caldocellum homologue for this second protein, we can detect no activity from this domain. |
en |
dc.language |
EN |
en |
dc.relation.ispartofseries |
Applied Microbiology and Biotechnology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0175-7598/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
SEQUENCE-ANALYSIS |
en |
dc.subject |
NUCLEOTIDE-SEQUENCE |
en |
dc.subject |
CLOSTRIDIUM-THERMOCELLUM |
en |
dc.subject |
TRICHODERMA-REESEI |
en |
dc.subject |
ESCHERICHIA-COLI |
en |
dc.subject |
BETA-MANNANASE |
en |
dc.subject |
CLONING |
en |
dc.subject |
EXPRESSION |
en |
dc.subject |
CLUSTER |
en |
dc.subject |
FAMILIES |
en |
dc.title |
celA, Another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s002530050405 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
291 |
en |
pubs.volume |
43 |
en |
dc.rights.holder |
Copyright: 1995 Springer Verlag |
en |
dc.identifier.pmid |
7612247 |
en |
pubs.end-page |
296 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
185420 |
en |
pubs.record-created-at-source-date |
2013-06-05 |
en |
pubs.dimensions-id |
7612247 |
en |