dc.contributor.author |
Gibbs, MD |
en |
dc.contributor.author |
Nevalainen, KMH |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.date.accessioned |
2011-07-24T21:41:21Z |
en |
dc.date.issued |
2001-06-13 |
en |
dc.identifier.citation |
Gene 271(1):13-20 13 Jun 2001 |
en |
dc.identifier.issn |
0378-1119 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7037 |
en |
dc.description.abstract |
Improvement of the biochemical characteristics of enzymes has been aided by misincorporation mutagenesis and DNA shuffling. Shuffling techniques can be used on a collection of mutants of the same gene, or related families of genes can be shuffled to produce mutants encoding chimeric gene products. One difficulty with current shuffling procedures is the predominance of unshuffled ('parental') molecules in the pool of mutants. We describe a procedure for gene shuffling using degenerate primers that allows control of the relative levels of recombination between the genes that are shuffled and reduces the regeneration of unshuffled parental genes. This procedure has the advantage of avoiding the use of endonucleases for gene fragmentation prior to shuffling and allows the use of random mutagenesis of selected segments of the gene as part of the procedure. We illustrate the use of the technique with a diverse family of P-xylanase genes that possess widely different G + C contents. |
en |
dc.language |
English |
en |
dc.publisher |
ELSEVIER SCIENCE BV |
en |
dc.relation.ispartofseries |
GENE |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0378-1119/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Genetics & Heredity |
en |
dc.subject |
gene shuffling |
en |
dc.subject |
xylanase genes |
en |
dc.subject |
overlap extension PCR |
en |
dc.subject |
BACILLUS-CIRCULANS XYLANASE |
en |
dc.subject |
DICTYOGLOMUS-THERMOPHILUM RT46B.1 |
en |
dc.subject |
DIRECTED EVOLUTION |
en |
dc.subject |
IN-VITRO |
en |
dc.subject |
MOLECULAR EVOLUTION |
en |
dc.subject |
BINDING DOMAINS |
en |
dc.subject |
KRAFT PULP |
en |
dc.subject |
DNA |
en |
dc.subject |
IDENTIFICATION |
en |
dc.subject |
SEQUENCE |
en |
dc.title |
Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/S0378-1119(01)00506-6 |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
13 |
en |
pubs.volume |
271 |
en |
dc.rights.holder |
Copyright: 2001 Elsevier Science B.V. |
en |
dc.identifier.pmid |
11410361 |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000170329200002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.end-page |
20 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
197359 |
en |
pubs.record-created-at-source-date |
2013-06-05 |
en |
pubs.dimensions-id |
11410361 |
en |