dc.contributor.author |
Bergquist, Peter |
en |
dc.contributor.author |
Sowden, Elizabeth |
en |
dc.contributor.author |
Ferrari, BC |
en |
dc.contributor.author |
Winsley, T |
en |
dc.date.accessioned |
2011-07-24T21:41:41Z |
en |
dc.date.issued |
2009-05 |
en |
dc.identifier.citation |
Extremophiles 13(3):389-401 May 2009 |
en |
dc.identifier.issn |
1431-0651 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7038 |
en |
dc.description.abstract |
Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-inwater emulsions or with individual genes in transcriptional/ translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis. |
en |
dc.language |
EN |
en |
dc.publisher |
SPRINGER TOKYO |
en |
dc.relation.ispartofseries |
EXTREMOPHILES |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1431-0651/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Fluorescence-activated cell sorting |
en |
dc.subject |
Directed evolution |
en |
dc.subject |
In vitro compartmentalisation |
en |
dc.subject |
Non-culturable bacteria |
en |
dc.subject |
Single cell analysis |
en |
dc.subject |
Random (neutral) drift |
en |
dc.subject |
Random mutagenesis |
en |
dc.subject |
Protein optimisation |
en |
dc.subject |
IN-VITRO COMPARTMENTALIZATION |
en |
dc.subject |
WHOLE-CELL BIOSENSOR |
en |
dc.subject |
DIRECTED EVOLUTION |
en |
dc.subject |
MOLECULAR ANALYSIS |
en |
dc.subject |
ENZYME EVOLUTION |
en |
dc.subject |
DOUBLE EMULSIONS |
en |
dc.subject |
QUANTUM DOTS |
en |
dc.subject |
FLUORESCENCE |
en |
dc.subject |
LIBRARIES |
en |
dc.subject |
BACTERIA |
en |
dc.title |
Applications of flow cytometry in environmental microbiology and biotechnology |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00792-009-0236-4 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
389 |
en |
pubs.volume |
13 |
en |
dc.rights.holder |
Copyright: 2009 Springer |
en |
dc.identifier.pmid |
19301090 |
en |
pubs.end-page |
401 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Review |
en |
pubs.elements-id |
197348 |
en |
pubs.org-id |
Libraries & Learning Services |
en |
pubs.org-id |
Libraries & Learning Services |
en |
pubs.org-id |
Research and Collections |
en |
pubs.org-id |
Research and Collections |
en |
pubs.org-id |
Research Services |
en |
pubs.org-id |
Research Services |
en |
pubs.record-created-at-source-date |
2011-11-02 |
en |
pubs.dimensions-id |
19301090 |
en |