Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

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dc.contributor.author Ferrari, BC en
dc.contributor.author Power, ML en
dc.contributor.author Bergquist, Peter en
dc.date.accessioned 2011-07-24T21:43:04Z en
dc.date.issued 2007-12 en
dc.identifier.citation Biotechnol Lett 29(12):1831-1837 Dec 2007 en
dc.identifier.issn 0141-5492 en
dc.identifier.uri http://hdl.handle.net/2292/7044 en
dc.description.abstract Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for crosscontamination. We describe here a closed-tube DNA extraction procedure based upon the use of a thermostable proteinase that enabled sensitive amplification of target loci from parasites from diverse lineages including Apicomplexa, Sarcomastgophora and Nematoda. Moreover, this procedure is not subject to cross-contamination and is readily adaptable to automation. en
dc.language EN en
dc.publisher SPRINGER en
dc.relation.ispartofseries BIOTECHNOL LETT en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0141-5492/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject DNA extraction en
dc.subject cryptosporidium en
dc.subject giardia en
dc.subject haemonchus en
dc.subject nematoda en
dc.subject proteinase en
dc.subject protozoa en
dc.subject CRYPTOSPORIDIUM-PARVUM OOCYSTS en
dc.subject CHAIN-REACTION DETECTION en
dc.subject REAL-TIME PCR en
dc.subject BENZIMIDAZOLE RESISTANCE en
dc.subject GIARDIA-LAMBLIA en
dc.subject HUMANS en
dc.subject GENOTYPES en
dc.subject SAMPLES en
dc.subject ASSAY en
dc.title Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection en
dc.type Journal Article en
dc.identifier.doi 10.1007/s10529-007-9487-2 en
pubs.issue 12 en
pubs.begin-page 1831 en
pubs.volume 29 en
dc.rights.holder Copyright: 2007 Springer Science+Business Media B.V. en
dc.identifier.pmid 17657409 en
pubs.end-page 1837 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 197351 en
pubs.record-created-at-source-date 2011-11-02 en
pubs.dimensions-id 17657409 en


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