GENES FOR PHASEOLOTOXIN SYNTHESIS ARE LOCATED ON THE CHROMOSOME OF PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA

QUIGLEY, NB; LANE, D; Bergquist, Peter

dc.contributor.author	QUIGLEY, NB	en
dc.contributor.author	LANE, D	en
dc.contributor.author	Bergquist, Peter	en
dc.date.accessioned	2011-07-24T21:43:17Z	en
dc.date.issued	1985-01-01	en
dc.identifier.citation	Current Microbiology 12(5):295-299 1985	en
dc.identifier.issn	0343-8651	en
dc.identifier.uri	http://hdl.handle.net/2292/7045	en
dc.description.abstract	Transposon mutagenesis was used to locate the genes for phaseolotoxin biosynthesis in Pseudomonas syringae pathovar, phaseolicola. Mutants unable to produce toxin were obtained that carried Tn5 on different chromosomal restriction fragments. None of the Tn5-induced nontoxigenic mutants carried the transposon in plasmid DNA. The insertion of Tn5 into tox DNA was confirmed by site-directed mutagenesis. The results reported here suggest the involvement of at least five chromosomal genes in phaseolotoxin biosynthesis. All of the toxinminus mutants retained both full pathogenicity on beans and resistance to the toxin.	en
dc.language	English	en
dc.publisher	SPRINGER VERLAG	en
dc.relation.ispartofseries	CURRENT MICROBIOLOGY	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0343-8651/	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.subject	Science & Technology	en
dc.subject	Life Sciences & Biomedicine	en
dc.subject	Microbiology	en
dc.title	GENES FOR PHASEOLOTOXIN SYNTHESIS ARE LOCATED ON THE CHROMOSOME OF PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA	en
dc.type	Journal Article	en
dc.identifier.doi	10.1007/BF01567981	en
pubs.issue	5	en
pubs.begin-page	295	en
pubs.volume	12	en
dc.rights.holder	Copyright: 1985 Springer-Verlag	en
pubs.author-url	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1985AXA0600010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d	en
pubs.end-page	299	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/RestrictedAccess	en
pubs.subtype	Article	en
pubs.elements-id	197384	en
pubs.record-created-at-source-date	2013-06-05	en