dc.contributor.author |
LUTHI, E |
en |
dc.contributor.author |
JASMAT, NB |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.date.accessioned |
2011-07-24T21:44:30Z |
en |
dc.date.issued |
1990-11-01 |
en |
dc.identifier.citation |
Appl Microbiol Biotechnol 34(2):214-219 Nov 1990 |
en |
dc.identifier.issn |
0175-7598 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7051 |
en |
dc.description.abstract |
The xynC gene coding for an acetylxylan esterase from the extreme thermophile 'Caldocellum saccharolyticum' was overexpressed in Escherichia coli strain RR28 by cloning the gene downstream from the lacZ promoter region of pUC18 (pNZ1447) or downstream from the temperature-inducible γp(R)p(L) promoters of pJLA602 (pNZ1600). The protein formed high molecular weight aggregates in induced cells of RR28/pNZ1600 but not in RR28/pNZ1447. The enzyme constituted up to 10% of the total cell protein and was located in the cytoplasmic fraction of RR28/pNZ1447. The acetyl esterase as most active at pH 6.0 and 70-75°C with a half-life of 64 h at 70°C and 30 h at 80°C, respectively. |
en |
dc.language |
English |
en |
dc.publisher |
SPRINGER VERLAG |
en |
dc.relation.ispartofseries |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0175-7598/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Biotechnology & Applied Microbiology |
en |
dc.title |
OVERPRODUCTION OF AN ACETYLXYLAN ESTERASE FROM THE EXTREME THERMOPHILE CALDOCELLUM-SACCHAROLYTICUM IN ESCHERICHIA-COLI |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/BF00166783 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
214 |
en |
pubs.volume |
34 |
en |
dc.rights.holder |
Copyright: 1990 Springer-Verlag |
en |
dc.identifier.pmid |
1367035 |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=A1990EK71100014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.end-page |
219 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
197376 |
en |
pubs.record-created-at-source-date |
2013-06-05 |
en |
pubs.dimensions-id |
1367035 |
en |