dc.contributor.author |
Bell, PJL |
en |
dc.contributor.author |
Nevalainen, H |
en |
dc.contributor.author |
Morgan, HW |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.date.accessioned |
2011-07-24T21:44:41Z |
en |
dc.date.issued |
1999 |
en |
dc.identifier.citation |
Biotechnology Letters 21:1003-1006 1999 |
en |
dc.identifier.issn |
0141-5492 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7052 |
en |
dc.description.abstract |
A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with t |
en |
dc.language |
EN |
en |
dc.relation.ispartofseries |
Biotechnology Letters |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0141-5492/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Bacillus |
en |
dc.subject |
gene cloning |
en |
dc.subject |
lipase |
en |
dc.subject |
thermophile |
en |
dc.subject |
NUCLEOTIDE-SEQUENCE |
en |
dc.subject |
THERMOSTABLE LIPASE |
en |
dc.subject |
BACTERIAL LIPASES |
en |
dc.subject |
GENE |
en |
dc.title |
Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1023/A:1005654701905 |
en |
pubs.issue |
11 |
en |
pubs.begin-page |
1003 |
en |
pubs.volume |
21 |
en |
dc.rights.holder |
Copyright: 1999 Kluwer Academic Publishers |
en |
pubs.end-page |
1006 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
1997 |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |