dc.contributor.author |
Pinheiro, LB |
en |
dc.contributor.author |
Gibbs, MD |
en |
dc.contributor.author |
Vesey, G |
en |
dc.contributor.author |
Smith, JJ |
en |
dc.contributor.author |
Bergquist, Peter |
en |
dc.date.accessioned |
2011-07-24T21:48:46Z |
en |
dc.date.issued |
2008-01 |
en |
dc.identifier.citation |
APPL MICROBIOL BIOT 77(6):1287-1295 Jan 2008 |
en |
dc.identifier.issn |
0175-7598 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7079 |
en |
dc.description.abstract |
Fluorescent reference strains of bacteria carrying a stable chromosomally integrated single copy of the gfp gene have been developed. A modified version of the gfp gene has been generated by mutagenesis and expressed under the control of the bacteriophage lambda promoter PL. A cassette comprising bacteriophage Mu transposon arms flanking the modified gfp gene and regulatory regions was irreversibly integrated as an in-vitro-assembled transposition complex into the genomes of Escherichia coli and Salmonella spp. The modified green fluorescent protein (GFP) protein retained the fluorescence excitation and emission wavelengths of wild-type GFP. However, it fluoresced more brightly in E. coli and Salmonella compared to wild-type GFP, presumably due to improved protein maturation. Fluorescent E. coli and Salmonella strains carrying the gfp gene cassette were easily differentiated from their respective non-fluorescent parental strains on various growth media by visualization under UV light. The bacterial strains produced by this method remained viable and stably fluorescent when incorporated into a matrix for delivery of exact numbers of viable bacterial cells for use as quality control agents in microbiological procedures. |
en |
dc.language |
EN |
en |
dc.publisher |
SPRINGER |
en |
dc.relation.ispartofseries |
APPL MICROBIOL BIOT |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0175-7598/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
GRAM-NEGATIVE BACTERIA |
en |
dc.subject |
LACTIC-ACID BACTERIA |
en |
dc.subject |
ESCHERICHIA-COLI |
en |
dc.subject |
TRANSPOSITION COMPLEXES |
en |
dc.subject |
MARKER |
en |
dc.subject |
GFP |
en |
dc.subject |
SURVIVAL |
en |
dc.subject |
MUTAGENESIS |
en |
dc.subject |
EXPRESSION |
en |
dc.subject |
VECTORS |
en |
dc.title |
Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1007/s00253-007-1253-9 |
en |
pubs.issue |
6 |
en |
pubs.begin-page |
1287 |
en |
pubs.volume |
77 |
en |
dc.rights.holder |
Copyright: 2007 Springer-Verlag |
en |
dc.identifier.pmid |
17994234 |
en |
pubs.end-page |
1295 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
114671 |
en |
pubs.record-created-at-source-date |
2011-11-02 |
en |
pubs.dimensions-id |
17994234 |
en |