Potential Use of Quantum Dots in Flow Cytometry

Ibanez-Peral, R; Bergquist, Peter; Walter, MR; Gibbs, M; Goldys, EM; Ferrari, B

dc.contributor.author	Ibanez-Peral, R	en
dc.contributor.author	Bergquist, Peter	en
dc.contributor.author	Walter, MR	en
dc.contributor.author	Gibbs, M	en
dc.contributor.author	Goldys, EM	en
dc.contributor.author	Ferrari, B	en
dc.date.accessioned	2011-07-24T21:49:44Z	en
dc.date.issued	2008-12	en
dc.identifier.citation	INT J MOL SCI 9(12):2622-2638 Dec 2008	en
dc.identifier.issn	1422-0067	en
dc.identifier.uri	http://hdl.handle.net/2292/7085	en
dc.description.abstract	QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.	en
dc.language	EN	en
dc.publisher	MOLECULAR DIVERSITY PRESERVATION INT	en
dc.relation.ispartofseries	INT J MOL SCI	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1422-0067//	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/	en
dc.subject	Quantum dots	en
dc.subject	flow cytometry	en
dc.subject	fluorescent detection	en
dc.subject	detection limit	en
dc.subject	autofluorescence	en
dc.subject	CRYPTOSPORIDIUM-PARVUM	en
dc.subject	IN-VIVO	en
dc.subject	CELLS	en
dc.subject	NANOCRYSTALS	en
dc.subject	TRACKING	en
dc.subject	SYSTEM	en
dc.subject	BEADS	en
dc.subject	ASSAY	en
dc.subject	SIZE	en
dc.title	Potential Use of Quantum Dots in Flow Cytometry	en
dc.type	Journal Article	en
dc.identifier.doi	10.3390/ijms9122622	en
pubs.issue	12	en
pubs.begin-page	2622	en
pubs.volume	9	en
dc.rights.holder	Copyright: the author	en
dc.identifier.pmid	19330097	en
pubs.end-page	2638	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/OpenAccess	en
pubs.subtype	Article	en
pubs.elements-id	197349	en
pubs.record-created-at-source-date	2011-11-02	en
pubs.dimensions-id	19330097	en