Changes in the calcium current among different transmural regions contributes to action potential heterogeneity in rat heart.

Show simple item record Kim, N en Cannell, Mark en Hunter, Peter en
dc.coverage.spatial England en 2011-08-16T03:37:33Z en 2010-09 en
dc.identifier.citation Prog Biophys Mol Biol 103(1):28-34 Sep 2010 en
dc.identifier.issn 0079-6107 en
dc.identifier.uri en
dc.description.abstract To clarify the transmural heterogeneity of action potential (AP) time course, we examined the regulation of L-type Ca(2+) current (I(Ca,L)) by voltage and Ca(2+)-dependent mechanisms. Currents were recorded using patch clamp of single rat subepicardial (EPI) and subendocardial (ENDO) of left ventricular, right ventricular (RV) and septal (SEP) cardiomyocytes. Voltage clamp commands were derived from ENDO and EPI APs or rectangular voltage pulses. During rectangular pulses, peak I(Ca,L) was significantly greater in EPI than in other cells. The inactivation of I(Ca,L) by Ca(2+)-dependent mechanisms (suppressed by ryanodine and BAPTA) was present in all cells but greater in extent in ENDO and SEP cells. Activation and inactivation curves for all regions show subtle differences that are Ca(2+) sensitive, with Ca(2+) inactivation shifting the activation variables negative by approximately 7 mV and inactivation variables positive by 2-7 mV (EPI being least, RV greatest). In AP-clamps, the peak I(Ca,L) was significantly smaller in ENDO than in EPI cells, while the integrated current was significantly larger in ENDO than in EPI cells. The results are discussed with regard to the interplay of AP time course and net Ca(2+) influx. en
dc.language eng en
dc.relation.ispartofseries Prog Biophys Mol Biol en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.subject Action Potentials en
dc.subject Animals en
dc.subject Calcium en
dc.subject Heart en
dc.subject Myocytes, Cardiac en
dc.subject Patch-Clamp Techniques en
dc.subject Rats en
dc.subject Ventricular Function en
dc.title Changes in the calcium current among different transmural regions contributes to action potential heterogeneity in rat heart. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.pbiomolbio.2010.05.004 en
pubs.issue 1 en
pubs.begin-page 28 en
pubs.volume 103 en
dc.rights.holder Copyright: 2010 Elsevier Ltd. en
dc.identifier.pmid 20553743 en
pubs.end-page 34 en
dc.rights.accessrights en
pubs.subtype Review en
pubs.elements-id 120198 en Bioengineering Institute en ABI Associates en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1873-1732 en
dc.identifier.pii S0079-6107(10)00032-5 en
pubs.record-created-at-source-date 2011-10-21 en
pubs.dimensions-id 20553743 en

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