dc.contributor.author |
Ghajar, CM |
en |
dc.contributor.author |
Suresh, Vinod |
en |
dc.contributor.author |
Peyton, SR |
en |
dc.contributor.author |
Raub, CB |
en |
dc.contributor.author |
Meyskens, FL |
en |
dc.contributor.author |
George, SC |
en |
dc.contributor.author |
Putnam, AJ |
en |
dc.date.accessioned |
2011-09-04T21:09:33Z |
en |
dc.date.issued |
2007 |
en |
dc.identifier.citation |
Mol Cancer Ther 6(2):552-561 Feb 2007 |
en |
dc.identifier.issn |
1535-7163 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/7552 |
en |
dc.description.abstract |
Although attempts to develop any viable chemotherapeutic approaches to combat metastatic cancers have largely failed, potential genetic targets to halt metastatic progression continue to be identified. As drugs are developed to address these targets, there is a need for high-throughput systems that accurately reproduce in vivo microenvironments to gauge their efficacy. Accordingly, we have developed a three-dimensional in vitro culture system representative of the environment present upon secondary metastasis to quantitatively measure tumor cell invasion in this setting three-dimensionally. Culturing melanomas of different metastatic capacities within the system showed that each cell type invades the matrix in a manner commensurate to its known metastatic potential in vivo. Moreover, the developed quantitative schemes were put to use to characterize the effect of microenvironmental influences (i.e., matrix components, interstitial cell presence) on planar and vertical melanoma invasion. We propose this novel, quantitative system as a useful tool to assess the effects of pharmacologic and/or microenvironmental influences on tumor cell invasion at a metastatic site. |
en |
dc.language |
EN |
en |
dc.relation.ispartofseries |
Molecular Cancer Therapeutics |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1535-7163/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
TUMOR-CELL INVASION |
en |
dc.subject |
BIOLOGICAL SIGNIFICANCE |
en |
dc.subject |
MALIGNANT-MELANOMA |
en |
dc.subject |
SKIN RECONSTRUCTS |
en |
dc.subject |
IN-VITRO |
en |
dc.subject |
CANCER |
en |
dc.subject |
FIBRIN |
en |
dc.subject |
PROGRESSION |
en |
dc.subject |
MICROENVIRONMENT |
en |
dc.subject |
MATRIX |
en |
dc.title |
A novel three-dimensional model to quantify metastatic melanoma invasion |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1158/1535-7163.MCT-06-0593 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
552 |
en |
pubs.volume |
6 |
en |
dc.rights.holder |
Copyright: 2007 American Association for Cancer Research |
en |
dc.identifier.pmid |
17267658 |
en |
pubs.author-url |
http://mct.aacrjournals.org/content/6/2/552.abstract |
en |
pubs.end-page |
561 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
80855 |
en |
pubs.org-id |
Bioengineering Institute |
en |
pubs.org-id |
ABI Associates |
en |
pubs.org-id |
Engineering |
en |
pubs.org-id |
Engineering Science |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
17267658 |
en |