Three-Dimensional Structural Characterization of Tissue Engineered and Native Ovine Pulmonary Valves

Show simple item record Eckert, Chad en Gerneke, Dane en Le Grice, Ian en Gottlieb, David en Mayer, JE en Sacks, MS en
dc.coverage.spatial San Antonio, TX en 2011-09-06T22:20:54Z en 2009-04 en
dc.identifier.citation Society for Biomaterials 2009 Annual Meeting. Apr 2009 en
dc.identifier.uri en
dc.description.abstract OBJECTIVES: Efforts in tissue-engineered heart valves (TEHV) have shown increasingly equivalent mechanical/structural properties compared to native valves, though a literature gap exists regarding detailed structural information. This work was performed to provide such data of implanted TEHV, the native pulmonary valve (PV), and pre-implant scaffold to better understand developing TEHV. METHODS: Dynamically-cultured in vivo samples (“pre-implant”) and ovine TEHV PV in vitro samples (“explant”) were produced based on previous techniques; ovine PVs were excised. Samples were stained with picrosirius red and resinmounted. Using extended-volume scanning laser confocal microscopy (EV-SLCM), 1.5 x 1.5 x 0.4 mm full-thickness samples were imaged at 1 pixel/μm in 1 μm Z-direction steps. Custom software was used to process and visualize samples. Collagen, cell nuclei, and scaffold volume fractions were quantified; scaffold fiber trajectory and length were tracked using custom software. RESULTS: In a scaffold representative volume (90 μm thick), 104 fibers were tracked with a mean fiber length of 137.94 μm 55.4 μm (Fig.1). A comparison between pre-implant and explant samples showed collagen volume fraction increasing from 76.6% to 85.9%, with nuclei and scaffold decreasing from 2.8% to 0.5% and from 5.9% to 0.8%, respectively. With the native collagen volume fraction measured at 70%, pre-implant and explant samples showed an increase in collagen. CONCLUSIONS: This work captured important differences between in vivo/in vitro TEHV constituents; it is the first known work to utilize EV-SLCM on TEHV. A comparison to the native valve showed structural differences that could impact longterm functionality and improve design. en
dc.relation.ispartof Society for Biomaterials 2009 Annual Meeting en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Three-Dimensional Structural Characterization of Tissue Engineered and Native Ovine Pulmonary Valves en
dc.type Conference Poster en
dc.rights.holder Copyright: the author en
dc.rights.accessrights en
pubs.elements-id 99624 en Bioengineering Institute en ABI Associates en Medical and Health Sciences en Medical Sciences en Physiology Division en
pubs.record-created-at-source-date 2010-09-01 en

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