Viscoelastic Modeling of highly hydrated laminin layers at homogeneous and nanostructured surfaces: Quantification of protein layer properties using QCM-D and SPR

Show simple item record Malmstrom Pendred, Jenny en Agheli, H en Kingshott, P en Sutherland, DS en 2011-09-27T01:04:00Z en 2007 en
dc.identifier.citation LANGMUIR 23(19):9760-9768 11 Sep 2007 en
dc.identifier.issn 0743-7463 en
dc.identifier.uri en
dc.description.abstract The adsorption of proteins at material surfaces is important in applications such as biomaterials, drug delivery, and diagnostics. The interaction of cells with artificial surfaces is mediated through adsorbed proteins, where the type of protein, amount, orientation, and conformation are of consequence for the cell response. Laminin, an important cell adhesive protein that is central in developmental biology, is studied by a combination of quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR) to characterize the adsorption of laminin on surfaces of different surface chemistries. The combination of these two techniques allows for the determination of the thickness and effective density of the protein layer as well as the adsorbed mass and viscoelastic properties. We also evaluate the capacity of QCM-D to be used as a quantitative technique on a nanostructured surface, where protein is adsorbed specifically in a nanopattern exploiting PLL-g-PEG as a protein-resistant background. We show that laminin forms a highly hydrated protein layer with different characteristics depending on the underlying substrate. Using a combination of QCM-D and atomic force microscopy (AFM) data from nanostructured surfaces, we model laminin and antibody binding to nanometer-scale patches. A higher amount of laminin was found to adsorb in a thicker layer of a lower effective density in nanopatches compared to equivalent homogeneous surfaces. These results suggest that modeling of QCM-D data of soft viscoelastic layers arranged in nanopatterns may be applied where an independent measure of the "dry" mass is known. en
dc.language EN en
dc.publisher American Chemical Society en
dc.relation.ispartofseries Langmuir en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.subject POLYMER SURFACES en
dc.subject FILM THICKNESS en
dc.subject CELL-ADHESION en
dc.subject COUPLED WATER en
dc.subject FREQUENCY en
dc.title Viscoelastic Modeling of highly hydrated laminin layers at homogeneous and nanostructured surfaces: Quantification of protein layer properties using QCM-D and SPR en
dc.type Journal Article en
dc.identifier.doi 10.1021/la701233y en
pubs.issue 19 en
pubs.begin-page 9760 en
pubs.volume 23 en
dc.rights.holder Copyright: 2007 American Chemical Society en
dc.identifier.pmid 17691829 en
pubs.end-page 9768 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 221674 en Engineering en Chemical and Materials Eng en
pubs.record-created-at-source-date 2011-08-31 en
pubs.dimensions-id 17691829 en

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