Abstract:
1. An in vitro protein synthesizing system was developed from a
brewer's yeast (Saccharomyces cerevisiae). It was characterized,
and some of the conditions necessary for optimal protein synthesis
established.
2. Only a small proportion (leas than 20%) of the ribosomal population
was active in polyU-directed polyphenylalanine synthesis. These active ribosomes probably derived from the in vivo 80g monomers and ribosomal
subunits with the polysomal ribosanes being inactive in the cell-free
system.