Evaluation of methodology for detection of human adenoviruses in wastewater, drinking water, stream water and recreational waters

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dc.contributor.author Dong, Y en
dc.contributor.author Kim, J en
dc.contributor.author Lewis, GD en
dc.date.accessioned 2011-11-02T19:15:25Z en
dc.date.available 2011-11-02T19:15:25Z en
dc.date.issued 2010 en
dc.identifier.citation Journal of Applied Microbiology 108(3):800-809 Mar 2010 en
dc.identifier.issn 1364-5072 en
dc.identifier.uri http://hdl.handle.net/2292/8540 en
dc.description.abstract Aims:  This study evaluates dialysis filtration and a range of PCR detection methods for identification and quantification of human adenoviruses in a range of environmental waters. Methods and Results:  Adenovirus was concentrated from large volumes (50–200 l) of environmental and potable water by hollow fibre microfiltration using commercial dialysis filters. By this method, an acceptable recovery of a seeded control bacteriophage MS2 from seawater (median 95·5%, range 36–98%, n = 5), stream water (median 84·7%, range 23–94%, n = 5) and storm water (median 59·5%, range 6·3–112%, n = 5) was achieved. Adenovirus detection using integrated cell culture PCR (ICC-PCR), direct PCR, nested PCR, real-time quantitative PCR (qPCR) and adenovirus group F-specific direct PCR was tested with PCR products sequenced for confirmation. Adenovirus was routinely detected from all water types by most methods, with ICC-PCR more sensitive than direct-nested PCR or qPCR. Group F adenovirus dominated in wastewater samples but was detected very infrequently in environmental waters. Conclusions and Implications:  Human adenoviruses (HAdv) proved relatively common in environmental and potable waters when assessed using an efficient concentration method and sensitive detection method. ICC-PCR proved most sensitive, could be used semiquantitatively and demonstrated virus infectivity but was time consuming and expensive. qPCR provided quantitative results but was c. ten-fold less sensitive than the best methods. en
dc.language EN en
dc.relation.ispartofseries Journal of Applied Microbiology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1364-5072/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.source.uri http://dx.doi.org/10.1111/j.1365-2672.2009.04477.x en
dc.subject adenovirus en
dc.subject drinking water en
dc.subject environmental water en
dc.subject integrated cell culture PCR en
dc.subject rapid techniques qPCR en
dc.subject water quality en
dc.subject REAL-TIME PCR en
dc.subject ENTERIC VIRUSES en
dc.subject TAP WATER en
dc.subject ENTEROVIRUSES en
dc.subject BACTERIOPHAGES en
dc.subject SHELLFISH en
dc.subject ASSAY en
dc.subject QUANTIFICATION en
dc.title Evaluation of methodology for detection of human adenoviruses in wastewater, drinking water, stream water and recreational waters en
dc.type Journal Article en
dc.identifier.doi 10.1111/j.1365-2672.2009.04477.x en
pubs.issue 3 en
pubs.begin-page 800 en
pubs.volume 108 en
dc.rights.holder Copyright: WILEY-BLACKWELL PUBLISHING, INC en
pubs.end-page 809 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.elements-id 118772 en
pubs.org-id Faculty of Science en
pubs.org-id Biological Sciences en

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