dc.contributor.author |
Chen, Qi |
en |
dc.contributor.author |
Stone, Peter |
en |
dc.contributor.author |
McCowan, Lesley |
en |
dc.contributor.author |
Chamley, Lawrence |
en |
dc.date.accessioned |
2011-11-04T02:36:15Z |
en |
dc.date.issued |
2006 |
en |
dc.identifier.citation |
Hypertension 47:116-121 2006 |
en |
dc.identifier.issn |
0194-911X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/8686 |
en |
dc.description.abstract |
It is hypothesized that preeclampsia is caused by factors from the placenta that induce endothelial cell activation. Trophoblasts are cells that may be shed from the placenta, then deported in the maternal blood, and finally become trapped in the pulmonary capillaries. The ultimate fate of deported trophoblasts is unknown, but to prevent clogging of the pulmonary circulation they must be cleared from the capillary beds. We examined the hypothesis that endothelial cells phagocytose deported trophoblasts and also examined the consequent effects of the trophoblasts on endothelial cells. Fluorescently labeled trophoblast–derived choriocarcinoma cells were induced to become apoptotic or necrotic and exposed to endothelial cell monolayers. Confocal microscopy demonstrated uptake of both apoptotic and necrotic trophoblasts, and this phagocytosis could be inhibited by cytochalasin B. Phagocytosis of necrotic but not apoptotic trophoblasts induced increased endothelial intercellular adhesion molecule 1 (ICAM-1) expression, as well as increased adhesion of monocytes to endothelial cell monolayers. Inhibiting the phosphatidylinositol 3-kinase and p38 mitogen-activated protein kinase pathways blocked both expression of ICAM-1 and phagocytosis, whereas inhibition of the P42/44 mitogen-activated protein kinase pathway blocked only ICAM-1 expression. This work suggests that endothelial cells can phagocytose deported trophoblasts and that the mechanism of trophoblast death (apoptotic or necrotic) could have major effects on the maternal vascular response to shed trophoblasts. |
en |
dc.language |
EN |
en |
dc.publisher |
American Heart Association, Inc |
en |
dc.relation.ispartofseries |
Hypertension |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0194-911X/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
endothelium |
en |
dc.subject |
cell adhesion molecules |
en |
dc.subject |
hypertension, pregnancy |
en |
dc.subject |
leukocytes |
en |
dc.subject |
NF-KAPPA-B |
en |
dc.subject |
STAPHYLOCOCCUS-AUREUS |
en |
dc.subject |
MATERNAL CIRCULATION |
en |
dc.subject |
ADHESION MOLECULE |
en |
dc.subject |
PREECLAMPSIA |
en |
dc.subject |
PREGNANCY |
en |
dc.subject |
DEPORTATION |
en |
dc.subject |
EXPRESSION |
en |
dc.subject |
BLOOD |
en |
dc.subject |
SYNCYTIOTROPHOBLAST |
en |
dc.title |
Phagocytosis of necrotic but not apoptotic trophoblasts induces endothelial cell activation. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1161/01.HYP.0000196731.56062.7c |
en |
pubs.issue |
1 |
en |
pubs.begin-page |
116 |
en |
pubs.volume |
47 |
en |
dc.rights.holder |
Copyright: 2005 American Heart Association |
en |
dc.identifier.pmid |
16344369 |
en |
pubs.end-page |
121 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
42900 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Obstetrics and Gynaecology |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
16344369 |
en |