Differential Localization of gamma-Aminobutyric Acid Type A and Glycine Receptor Subunits and Gephyrin in the Human Pons, Medulla Oblongata and Uppermost Cervical Segment of the Spinal Cord: An Immunohistochemical Study

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dc.contributor.author Waldvogel, Henry en
dc.contributor.author Baer, K en
dc.contributor.author Eady, E en
dc.contributor.author Allen, Kathryn en
dc.contributor.author Gilbert, Raymond en
dc.contributor.author Mohler, Hans en
dc.contributor.author Rees, Mark en
dc.contributor.author Nicholson, Louise en
dc.contributor.author Faull, Richard en
dc.date.accessioned 2011-11-17T17:19:22Z en
dc.date.issued 2010-02-01 en
dc.identifier.citation Journal of Comparative Neurology 518(3):305-328 01 Feb 2010 en
dc.identifier.issn 0021-9967 en
dc.identifier.uri http://hdl.handle.net/2292/9232 en
dc.description.abstract Gephyrin is a multifunctional protein responsible for the clustering of glycine receptors (GlyR) and gamma-aminobutyric acid type A receptors (GABA(A)R). GlyR and GABA(A)R are heteropentameric chloride ion channels that facilitate fast-response, inhibitory neurotransmission in the mammalian brain and spinal cord. We investigated the immunohistochemical distribution of gephyrin and the major GABA(A)R and GlyR subunits in the human light microscopically in the rostral and caudal one-thirds of the pons, in the middle and caudal one-thirds of the medulla oblongata, and in the first cervical segment of the spinal cord. The results demonstrate a widespread pattern of immunoreactivity for GlyR and GABA(A)R subunits throughout these regions, including the spinal trigeminal nucleus, abducens nucleus, facial nucleus, pontine reticular formation, dorsal motor nucleus of the vagus nerve, hypoglossal nucleus, lateral cuneate nucleus, and nucleus of the solitary tract. The GABA(A)R alpha(1), and GlyR alpha(1), and beta subunits show high levels of immunoreactivity in these nuclei. The GABA(A)R subunits alpha(2), alpha(3), beta(2,3), and gamma(2) present weaker levels of immunoreactivity. Exceptions are intense levels of GABA(A)R alpha(2) subunit immunoreactivity in the inferior olivary complex and high levels of GABA(A)R alpha(3) subunit immunoreactivity in the locus coeruleus and raphe nuclei. Gephyrin immunoreactivity is highest in the first segment of the cervical spinal cord and hypoglossal nucleus. Our results suggest that a variety of different inhibitory receptor subtypes is responsible for inhibitory functions in the human brainstem and cervical spinal cord and that gephyrin functions as a clustering molecule for major subtypes of these inhibitory neurotransmitter receptors. J. Comp. Neurol. 518:305-328, 2010. (C) 2009 Wiley-Liss, Inc. en
dc.language EN en
dc.publisher WILEY-LISS en
dc.relation.ispartofseries Journal of Comparative Neurology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0021-9967/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject human brain en
dc.subject brainstem en
dc.subject gephyrin en
dc.subject glycine receptor en
dc.subject GABA(A) receptor en
dc.subject immunohistochemistry en
dc.subject ADULT-RAT BRAIN en
dc.subject SUBSTANTIA-NIGRA en
dc.subject BETA-SUBUNIT en
dc.subject GLOBUS-PALLIDUS en
dc.subject ALPHA-1 SUBUNIT en
dc.title Differential Localization of gamma-Aminobutyric Acid Type A and Glycine Receptor Subunits and Gephyrin in the Human Pons, Medulla Oblongata and Uppermost Cervical Segment of the Spinal Cord: An Immunohistochemical Study en
dc.type Journal Article en
dc.identifier.doi 10.1002/cne.22212 en
pubs.issue 3 en
pubs.begin-page 305 en
pubs.volume 518 en
dc.rights.holder Copyright: Wiley-Liss, Inc. en
dc.identifier.pmid 19950251 en
pubs.end-page 328 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 86579 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Anatomy and Medical Imaging en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 19950251 en

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